Hordoindolines are predominantly expressed in the aleurone layer in late kernel development in barley

So Lee Mi, Seong Jang Cheol, Shin Lee Sung, Yoon Kim Jae, Moo Lee Byoung, Chun Seong Rak, Yong Weon Seo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to isolate grain-specific genes during kernel development, two differential screening methods - suppression subtractive hybridization (SSH) and differential hybridization (DH) - were applied. Two cDNAs encoding hordoindolines known to be related to grain hardness were isolated and designated as Hordeum vulgare indoline a and b (HvIDa and HvIDb). The cDNAs encoding HvIDa and HvIDb contained a 450 bp and a 444 bp open reading frame (ORF) that encoded the putative hordoindoline-a and -b precursors consisting of 150 and 148 amino acids, respectively. The deduced amino acid sequences of both HvIDa and HvIDb contained one tryptophan-rich domain and ten highly conserved cysteine residues. The expression of the HvIDa gene was high at 5 DAF, reached a peak at 8 DAF and decreased slightly until 20 DAF, while the expression of HvIDb began to be detectable at 8 DAF when it was higher than that of other developmental stages and decreased slightly until 20 DAF. The HvIDa and HvIDb genes were predominantly detected in the aleurone cell layers in the late part of kernel development, e.g. at 20 DAF. These findings may provide clues to the molecular mechanisms of kernel development and contribute to the determination of the grain texture in barley.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalBreeding Science
Volume56
Issue number1
DOIs
Publication statusPublished - 2006 Mar 21

Fingerprint

aleurone layer
Hordeum
seed development
Complementary DNA
barley
Hardness
Tryptophan
Open Reading Frames
Genes
aleurone cells
Cysteine
Amino Acid Sequence
suppression subtractive hybridization
Gene Expression
Amino Acids
tryptophan
Hordeum vulgare
cysteine
hardness
open reading frames

Keywords

  • Aleurone layer
  • Barley
  • Hordoindolines
  • Kernel development

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Hordoindolines are predominantly expressed in the aleurone layer in late kernel development in barley. / Mi, So Lee; Cheol, Seong Jang; Sung, Shin Lee; Jae, Yoon Kim; Byoung, Moo Lee; Rak, Chun Seong; Seo, Yong Weon.

In: Breeding Science, Vol. 56, No. 1, 21.03.2006, p. 63-68.

Research output: Contribution to journalArticle

Mi, So Lee ; Cheol, Seong Jang ; Sung, Shin Lee ; Jae, Yoon Kim ; Byoung, Moo Lee ; Rak, Chun Seong ; Seo, Yong Weon. / Hordoindolines are predominantly expressed in the aleurone layer in late kernel development in barley. In: Breeding Science. 2006 ; Vol. 56, No. 1. pp. 63-68.
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