Hormonal regulation of beta-catenin during development of the avian oviduct and its expression in epithelial cell-derived ovarian carcinogenesis

Seung Min Bae, Whasun Lim, Wooyoung Jeong, Jin Young Lee, Jinyoung Kim, Jae Yong Han, Fuller W. Bazer, Gwonhwa Song

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Beta-catenin (CTNNB1) is a dual function molecule that acts as a key component of the cadherin complex and WNT signaling pathway. It has a crucial role in embryogenesis, tumorigenesis, angiogenesis and progression of metastasis. Recently, it has been suggested that the CTNNB1 complex is a major regulator of development of the mouse oviduct and uterus. However, little is known about the CTNNB1 gene in chickens. Therefore, in this study, we focused on the CTNNB1 gene in the chicken reproductive tract and hormonal control of its expression in the chicken oviduct. CTNNB1 was localized specifically to the luminal and glandular epithelium of the four segments of chicken oviduct and DES (diethylstilbestrol, a synthetic non-steroidal estrogen) increased its expression primarily in LE of the magnum. In addition, CTNNB1 mRNA and protein were expressed abundantly in glandular epithelium of endometrioid-type ovarian carcinoma, but not in normal ovaries. Moreover, CTNNB1 expression was post-transcriptionally regulated via its 3'-UTR by binding with target miRNAs including miR-217, miR-1467, miR-1623 and miR-1697. Collectively, these results indicate that CTNNB1 is a novel gene regulated by estrogen in epithelial cells of the chicken oviduct and that it is also abundantly expressed in epithelial cells of endometrioid-type ovarian carcinoma suggesting that it could be used as a marker for diagnosis of ovarian cancer in laying hens and women.

Original languageEnglish
Pages (from-to)46-54
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume382
Issue number1
DOIs
Publication statusPublished - 2014 Jan 25

Fingerprint

Oviducts
beta Catenin
Chickens
Carcinogenesis
Genes
Epithelial Cells
Endometrioid Carcinoma
Non-Steroidal Estrogens
Diethylstilbestrol
3' Untranslated Regions
Cadherins
MicroRNAs
Epithelium
Estrogens
Messenger RNA
Molecules
Ovarian Neoplasms
Uterus
Embryonic Development
Ovary

Keywords

  • Cancer
  • Chicken
  • CTNNB1
  • Estrogen
  • Ovary
  • Oviduct

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Hormonal regulation of beta-catenin during development of the avian oviduct and its expression in epithelial cell-derived ovarian carcinogenesis. / Bae, Seung Min; Lim, Whasun; Jeong, Wooyoung; Lee, Jin Young; Kim, Jinyoung; Han, Jae Yong; Bazer, Fuller W.; Song, Gwonhwa.

In: Molecular and Cellular Endocrinology, Vol. 382, No. 1, 25.01.2014, p. 46-54.

Research output: Contribution to journalArticle

Bae, Seung Min ; Lim, Whasun ; Jeong, Wooyoung ; Lee, Jin Young ; Kim, Jinyoung ; Han, Jae Yong ; Bazer, Fuller W. ; Song, Gwonhwa. / Hormonal regulation of beta-catenin during development of the avian oviduct and its expression in epithelial cell-derived ovarian carcinogenesis. In: Molecular and Cellular Endocrinology. 2014 ; Vol. 382, No. 1. pp. 46-54.
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