HOS5 - A negative regulator of osmotic stress-induced gene expression in Arabidopsis thaliana

Liming Xiong, Manabu Ishitani, Hojoung Lee, Jian Kang Zhu

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Osmotic stress activates the expression of many plant genes through ABA-dependent as well as ABA-independent signaling pathways. We report here the characterization of a novel mutant of Arabidopsis thaliana, hos5-1, which exhibits increased expression of the osmotic stress responsive RD29A gene. The expression of several other stress genes are also enhanced by the hos5-1 mutation. The enhanced expression is specific to ABA and osmotic stress because low temperature regulation of these genes is not altered in the mutant. Genetic analysis indicated that hos5-1 is a recessive mutation in a single nuclear gene on chromosome III. Double mutant analysis of hos5-1 and the ABA-deficient aba1-1 as well as the ABA-insensitive abi1-1 mutant indicated that the osmotic stress hypersensitivity of hos5-1 is not affected by ABA deficiency or insensitivity. Furthermore, combined treatments of hos5-1 with ABA and osmotic stress had an additive effect on RD29A-LUC expression. These results suggest that the osmotic stress hypersensitivity in hos5-1 may be ABA-independent. The germination of hos5-1 seeds was more resistant to ABA. However, the hos5-1 mutation did not influence stomatal control and only slightly affected the regulation of growth and proline accumulation by ABA. The hos5-1 mutation reveals a negative regulator of osmotic stress-responsive gene expression shared by ABA-dependent and ABA-independent osmotic stress signaling pathways.

Original languageEnglish
Pages (from-to)569-578
Number of pages10
JournalPlant Journal
Volume19
Issue number5
DOIs
Publication statusPublished - 1999 Sep 1
Externally publishedYes

Fingerprint

Osmotic Pressure
osmotic stress
Arabidopsis
Arabidopsis thaliana
Gene Expression
gene expression
mutation
mutants
Mutation
hypersensitive response
genes
Genes
Hypersensitivity
Plant Genes
Germination
additive effect
Proline
genetic techniques and protocols
proline
Seeds

ASJC Scopus subject areas

  • Plant Science

Cite this

HOS5 - A negative regulator of osmotic stress-induced gene expression in Arabidopsis thaliana. / Xiong, Liming; Ishitani, Manabu; Lee, Hojoung; Zhu, Jian Kang.

In: Plant Journal, Vol. 19, No. 5, 01.09.1999, p. 569-578.

Research output: Contribution to journalArticle

Xiong, Liming ; Ishitani, Manabu ; Lee, Hojoung ; Zhu, Jian Kang. / HOS5 - A negative regulator of osmotic stress-induced gene expression in Arabidopsis thaliana. In: Plant Journal. 1999 ; Vol. 19, No. 5. pp. 569-578.
@article{ecfd77d269724a1bab14e8e4c3bddc5c,
title = "HOS5 - A negative regulator of osmotic stress-induced gene expression in Arabidopsis thaliana",
abstract = "Osmotic stress activates the expression of many plant genes through ABA-dependent as well as ABA-independent signaling pathways. We report here the characterization of a novel mutant of Arabidopsis thaliana, hos5-1, which exhibits increased expression of the osmotic stress responsive RD29A gene. The expression of several other stress genes are also enhanced by the hos5-1 mutation. The enhanced expression is specific to ABA and osmotic stress because low temperature regulation of these genes is not altered in the mutant. Genetic analysis indicated that hos5-1 is a recessive mutation in a single nuclear gene on chromosome III. Double mutant analysis of hos5-1 and the ABA-deficient aba1-1 as well as the ABA-insensitive abi1-1 mutant indicated that the osmotic stress hypersensitivity of hos5-1 is not affected by ABA deficiency or insensitivity. Furthermore, combined treatments of hos5-1 with ABA and osmotic stress had an additive effect on RD29A-LUC expression. These results suggest that the osmotic stress hypersensitivity in hos5-1 may be ABA-independent. The germination of hos5-1 seeds was more resistant to ABA. However, the hos5-1 mutation did not influence stomatal control and only slightly affected the regulation of growth and proline accumulation by ABA. The hos5-1 mutation reveals a negative regulator of osmotic stress-responsive gene expression shared by ABA-dependent and ABA-independent osmotic stress signaling pathways.",
author = "Liming Xiong and Manabu Ishitani and Hojoung Lee and Zhu, {Jian Kang}",
year = "1999",
month = "9",
day = "1",
doi = "10.1046/j.1365-313X.1999.00558.x",
language = "English",
volume = "19",
pages = "569--578",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - HOS5 - A negative regulator of osmotic stress-induced gene expression in Arabidopsis thaliana

AU - Xiong, Liming

AU - Ishitani, Manabu

AU - Lee, Hojoung

AU - Zhu, Jian Kang

PY - 1999/9/1

Y1 - 1999/9/1

N2 - Osmotic stress activates the expression of many plant genes through ABA-dependent as well as ABA-independent signaling pathways. We report here the characterization of a novel mutant of Arabidopsis thaliana, hos5-1, which exhibits increased expression of the osmotic stress responsive RD29A gene. The expression of several other stress genes are also enhanced by the hos5-1 mutation. The enhanced expression is specific to ABA and osmotic stress because low temperature regulation of these genes is not altered in the mutant. Genetic analysis indicated that hos5-1 is a recessive mutation in a single nuclear gene on chromosome III. Double mutant analysis of hos5-1 and the ABA-deficient aba1-1 as well as the ABA-insensitive abi1-1 mutant indicated that the osmotic stress hypersensitivity of hos5-1 is not affected by ABA deficiency or insensitivity. Furthermore, combined treatments of hos5-1 with ABA and osmotic stress had an additive effect on RD29A-LUC expression. These results suggest that the osmotic stress hypersensitivity in hos5-1 may be ABA-independent. The germination of hos5-1 seeds was more resistant to ABA. However, the hos5-1 mutation did not influence stomatal control and only slightly affected the regulation of growth and proline accumulation by ABA. The hos5-1 mutation reveals a negative regulator of osmotic stress-responsive gene expression shared by ABA-dependent and ABA-independent osmotic stress signaling pathways.

AB - Osmotic stress activates the expression of many plant genes through ABA-dependent as well as ABA-independent signaling pathways. We report here the characterization of a novel mutant of Arabidopsis thaliana, hos5-1, which exhibits increased expression of the osmotic stress responsive RD29A gene. The expression of several other stress genes are also enhanced by the hos5-1 mutation. The enhanced expression is specific to ABA and osmotic stress because low temperature regulation of these genes is not altered in the mutant. Genetic analysis indicated that hos5-1 is a recessive mutation in a single nuclear gene on chromosome III. Double mutant analysis of hos5-1 and the ABA-deficient aba1-1 as well as the ABA-insensitive abi1-1 mutant indicated that the osmotic stress hypersensitivity of hos5-1 is not affected by ABA deficiency or insensitivity. Furthermore, combined treatments of hos5-1 with ABA and osmotic stress had an additive effect on RD29A-LUC expression. These results suggest that the osmotic stress hypersensitivity in hos5-1 may be ABA-independent. The germination of hos5-1 seeds was more resistant to ABA. However, the hos5-1 mutation did not influence stomatal control and only slightly affected the regulation of growth and proline accumulation by ABA. The hos5-1 mutation reveals a negative regulator of osmotic stress-responsive gene expression shared by ABA-dependent and ABA-independent osmotic stress signaling pathways.

UR - http://www.scopus.com/inward/record.url?scp=0033198503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033198503&partnerID=8YFLogxK

U2 - 10.1046/j.1365-313X.1999.00558.x

DO - 10.1046/j.1365-313X.1999.00558.x

M3 - Article

VL - 19

SP - 569

EP - 578

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -