Abstract
Crowding is an inherent property of living systems in which biochemical processes occur in highly concentrated solutions of various finite-sized species of both low (molecular crowding) and high (macromolecular crowding) molecular weights. Is molecular crowding fundamentally different from macromolecular crowding? To answer this question, we use a femtosecond mid-infrared pump-probe technique with three vibrational probes in molecular (diethylene glycol) and macromolecular (polyethylene glycol) solutions. In less crowded media, both molecular and macromolecular crowders fail to affect the dynamics of interstitial bulk-like water molecules and those at the crowder/water interface. In highly crowded media, interstitial water dynamics strongly depends on molecular crowding, but macromolecular crowding does not alter the bulk-like hydration dynamics and has a modest crowding effect on water at the crowder/water interface. The results of this study provide a molecular level understanding of the structural and dynamic changes to water and the water-mediated cross-linking of crowders.
| Original language | English |
|---|---|
| Pages (from-to) | 6584-6592 |
| Number of pages | 9 |
| Journal | Journal of Physical Chemistry Letters |
| Volume | 9 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 2018 Nov 15 |
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ASJC Scopus subject areas
- Materials Science(all)
- Physical and Theoretical Chemistry
Cite this
How Molecular Crowding Differs from Macromolecular Crowding : A Femtosecond Mid-Infrared Pump-Probe Study. / Verma, Pramod Kumar; Kundu, Achintya; Cho, Minhaeng.
In: Journal of Physical Chemistry Letters, Vol. 9, No. 22, 15.11.2018, p. 6584-6592.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - How Molecular Crowding Differs from Macromolecular Crowding
T2 - A Femtosecond Mid-Infrared Pump-Probe Study
AU - Verma, Pramod Kumar
AU - Kundu, Achintya
AU - Cho, Minhaeng
PY - 2018/11/15
Y1 - 2018/11/15
N2 - Crowding is an inherent property of living systems in which biochemical processes occur in highly concentrated solutions of various finite-sized species of both low (molecular crowding) and high (macromolecular crowding) molecular weights. Is molecular crowding fundamentally different from macromolecular crowding? To answer this question, we use a femtosecond mid-infrared pump-probe technique with three vibrational probes in molecular (diethylene glycol) and macromolecular (polyethylene glycol) solutions. In less crowded media, both molecular and macromolecular crowders fail to affect the dynamics of interstitial bulk-like water molecules and those at the crowder/water interface. In highly crowded media, interstitial water dynamics strongly depends on molecular crowding, but macromolecular crowding does not alter the bulk-like hydration dynamics and has a modest crowding effect on water at the crowder/water interface. The results of this study provide a molecular level understanding of the structural and dynamic changes to water and the water-mediated cross-linking of crowders.
AB - Crowding is an inherent property of living systems in which biochemical processes occur in highly concentrated solutions of various finite-sized species of both low (molecular crowding) and high (macromolecular crowding) molecular weights. Is molecular crowding fundamentally different from macromolecular crowding? To answer this question, we use a femtosecond mid-infrared pump-probe technique with three vibrational probes in molecular (diethylene glycol) and macromolecular (polyethylene glycol) solutions. In less crowded media, both molecular and macromolecular crowders fail to affect the dynamics of interstitial bulk-like water molecules and those at the crowder/water interface. In highly crowded media, interstitial water dynamics strongly depends on molecular crowding, but macromolecular crowding does not alter the bulk-like hydration dynamics and has a modest crowding effect on water at the crowder/water interface. The results of this study provide a molecular level understanding of the structural and dynamic changes to water and the water-mediated cross-linking of crowders.
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UR - http://www.scopus.com/inward/citedby.url?scp=85056653054&partnerID=8YFLogxK
U2 - 10.1021/acs.jpclett.8b03153
DO - 10.1021/acs.jpclett.8b03153
M3 - Article
C2 - 30380875
AN - SCOPUS:85056653054
VL - 9
SP - 6584
EP - 6592
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
SN - 1948-7185
IS - 22
ER -