HPLC determination of sildenafil and its demethylated metabolite, UK-103,320, in cat plasma with solid-phase extraction for the pharmacokinetic study

Byung Hwa Jung, Du Geon Moon, Bong Chul Chung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A simple and reliable high performance liquid chromatographic (HPLC) assay method was developed for the simultaneous determination of sildenafil and its demethylated metabolite (UK-103,320) in cat plasma. Solid-phase extraction with a C18SepPak cartridge after deproteinization was used, and the extraction recoveries were over 85% in both sildenafil and UK-103,320. The coefficient of variation was less than 15% in the 50-1000 ng/ml range for both sildenafil and UK-103,320, indicating that this assay has adequate reproducibility. This method was used to perform pharmacokinetic studies following oral administration of sildenafil citrate. Moment analysis revealed that the elimination half-life (7.4 ± 2.1 hr) was similar to those reported in studies using [14C]-sildenafil and the AUC percentage of UK-103,320 to sildenafil was also similar to the results of a previous report. These results indicate that this convenient analytical method is readily applicable to pharmacokinetic studies of sildenafil and UK-103,320.

Original languageEnglish
JournalCanadian Journal of Analytical Sciences and Spectroscopy
Volume46
Issue number2
Publication statusPublished - 2001 Dec 1
Externally publishedYes

Fingerprint

Pharmacokinetics
metabolites
cats
Metabolites
solid phases
Plasmas
cartridges
Liquids
citrates
liquids
half life
elimination
recovery
moments
coefficients
Assays
UK 103320
Sildenafil Citrate
Recovery

Keywords

  • HPLC
  • Pharmacokinetic
  • Sildenafil
  • Solid phase extraction
  • UK-103,320

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Spectroscopy

Cite this

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title = "HPLC determination of sildenafil and its demethylated metabolite, UK-103,320, in cat plasma with solid-phase extraction for the pharmacokinetic study",
abstract = "A simple and reliable high performance liquid chromatographic (HPLC) assay method was developed for the simultaneous determination of sildenafil and its demethylated metabolite (UK-103,320) in cat plasma. Solid-phase extraction with a C18SepPak cartridge after deproteinization was used, and the extraction recoveries were over 85{\%} in both sildenafil and UK-103,320. The coefficient of variation was less than 15{\%} in the 50-1000 ng/ml range for both sildenafil and UK-103,320, indicating that this assay has adequate reproducibility. This method was used to perform pharmacokinetic studies following oral administration of sildenafil citrate. Moment analysis revealed that the elimination half-life (7.4 ± 2.1 hr) was similar to those reported in studies using [14C]-sildenafil and the AUC percentage of UK-103,320 to sildenafil was also similar to the results of a previous report. These results indicate that this convenient analytical method is readily applicable to pharmacokinetic studies of sildenafil and UK-103,320.",
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AU - Jung, Byung Hwa

AU - Moon, Du Geon

AU - Chung, Bong Chul

PY - 2001/12/1

Y1 - 2001/12/1

N2 - A simple and reliable high performance liquid chromatographic (HPLC) assay method was developed for the simultaneous determination of sildenafil and its demethylated metabolite (UK-103,320) in cat plasma. Solid-phase extraction with a C18SepPak cartridge after deproteinization was used, and the extraction recoveries were over 85% in both sildenafil and UK-103,320. The coefficient of variation was less than 15% in the 50-1000 ng/ml range for both sildenafil and UK-103,320, indicating that this assay has adequate reproducibility. This method was used to perform pharmacokinetic studies following oral administration of sildenafil citrate. Moment analysis revealed that the elimination half-life (7.4 ± 2.1 hr) was similar to those reported in studies using [14C]-sildenafil and the AUC percentage of UK-103,320 to sildenafil was also similar to the results of a previous report. These results indicate that this convenient analytical method is readily applicable to pharmacokinetic studies of sildenafil and UK-103,320.

AB - A simple and reliable high performance liquid chromatographic (HPLC) assay method was developed for the simultaneous determination of sildenafil and its demethylated metabolite (UK-103,320) in cat plasma. Solid-phase extraction with a C18SepPak cartridge after deproteinization was used, and the extraction recoveries were over 85% in both sildenafil and UK-103,320. The coefficient of variation was less than 15% in the 50-1000 ng/ml range for both sildenafil and UK-103,320, indicating that this assay has adequate reproducibility. This method was used to perform pharmacokinetic studies following oral administration of sildenafil citrate. Moment analysis revealed that the elimination half-life (7.4 ± 2.1 hr) was similar to those reported in studies using [14C]-sildenafil and the AUC percentage of UK-103,320 to sildenafil was also similar to the results of a previous report. These results indicate that this convenient analytical method is readily applicable to pharmacokinetic studies of sildenafil and UK-103,320.

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