Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules

Sun Hee Kim, So Hee Bang, So Yeong Kang, Ki Dae Park, Jun Ho Eom, Il Ung Oh, Si Hyung Yoo, Chan Wha Kim, Sun Young Baek

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Human amniotic membrane-derived stromal cells (hAMSC) are candidates for cell-based therapies. We examined the characteristics of hAMSC including the interaction between hAMSC and breast cancer cells, MCF-7, and MDA-MB-231. Human amniotic membrane-derived stromal cells showed typical MSC properties, including fibroblast-like morphology, surface antigen expression, and mesodermal differentiation. To investigate cell-cell interaction via secreted molecules, we cultured breast cancer cells in hAMSC-conditioned medium (hAMSC-CM) and analyzed their proliferation, migration, and secretome profiles. MCF-7 and MDA-MB-231 cells exposed to hAMSC-CM showed increased proliferation and migration. However, in hAMSC-CM, MCF-7 cells proliferated significantly faster than MDA-MB-231 cells. When cultured in hAMSC-CM, MCF-7 cells migrated faster than MDA-MB-231 cells. Two cell types showed different profiles of secreted factors. MCF-7 cells expressed much amounts of IL-8, GRO, and MCP-1 in hAMSC-CM. Human amniotic membrane-derived stromal cells interact with breast cancer cells through secreted molecules. Factors secreted by hAMSCs promote the proliferation and migration of MCF-7 breast cancer cells. For much safe cell-based therapies using hAMSC, it is necessary to study carefully about interaction between hAMSC and cancer cells.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalTissue and Cell
Volume47
Issue number1
DOIs
Publication statusPublished - 2015 Feb 1

Keywords

  • Amniotic membrane
  • MCF-7
  • MDA-MB-231
  • Secretion

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

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  • Cite this

    Kim, S. H., Bang, S. H., Kang, S. Y., Park, K. D., Eom, J. H., Oh, I. U., Yoo, S. H., Kim, C. W., & Baek, S. Y. (2015). Human amniotic membrane-derived stromal cells (hAMSC) interact depending on breast cancer cell type through secreted molecules. Tissue and Cell, 47(1), 10-16. https://doi.org/10.1016/j.tice.2014.10.003