Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells

Chan Woo Kim, Tae Hoon Lee, Keun Hyung Park, Sang-Yun Choi, Jiyoung Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalFEBS Letters
Volume586
Issue number3
DOIs
Publication statusPublished - 2012 Feb 3

Fingerprint

Lactoferrin
Endothelial cells
Intercellular Adhesion Molecule-1
Endothelial Cells
DNA
Gene Expression Regulation
Gene expression
Chromatin
Anti-Inflammatory Agents

Keywords

  • Endothelial cell
  • Inflammation
  • Intercellular adhesion molecule-1 (ICAM-1)
  • Lactoferrin
  • NF-κB

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells. / Kim, Chan Woo; Lee, Tae Hoon; Park, Keun Hyung; Choi, Sang-Yun; Kim, Jiyoung.

In: FEBS Letters, Vol. 586, No. 3, 03.02.2012, p. 229-234.

Research output: Contribution to journalArticle

Kim, Chan Woo ; Lee, Tae Hoon ; Park, Keun Hyung ; Choi, Sang-Yun ; Kim, Jiyoung. / Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells. In: FEBS Letters. 2012 ; Vol. 586, No. 3. pp. 229-234.
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