Human lactoferrin suppresses TNF-α-induced intercellular adhesion molecule-1 expression via competition with NF-κB in endothelial cells

Chan Woo Kim, Tae Hoon Lee, Keun Hyung Park, Sang Yun Choi, Jiyoung Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalFEBS Letters
Volume586
Issue number3
DOIs
Publication statusPublished - 2012 Feb 3

Keywords

  • Endothelial cell
  • Inflammation
  • Intercellular adhesion molecule-1 (ICAM-1)
  • Lactoferrin
  • NF-κB

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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