Human leucine zipper protein sLZIP induces migration and invasion of cervical cancer cells via expression of matrix metalloproteinase-9

Hyereen Kang, Sung Wuk Jang, Je Sang Ko

Research output: Contribution to journalArticle

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Abstract

Extracellular proteolysis mediates tissue homeostasis. In cancer, altered proteolysis leads to abnormal tumor growth, inflammation, tissue invasion, and metastasis. Matrix metalloproteinase-9 (MMP-9) represents one of the most prominent proteinases associated with inflammation and tumorigenesis. The recently identified human transcription factor sLZIP is a member of the leucine zipper transcription factor family. Although sLZIP is known to function in ligand-induced transactivation of the glucocorticoid receptor, its exact functions and target genes are not known. In this study, we investigated the role of sLZIP in MMP-9 expression and its involvement in cervical cancer development. Our results show that sLZIP increased the expression of MMP-9 at both the mRNA and protein levels and the proteolytic activity of MMP-9 in HeLa and SiHa cells. sLZIP also increased the transcriptional activity of MMP-9by binding directly to the cAMP-responsive element of the MMP-9promoter region. Involvement of sLZIP in MMP-9expression was further supported by the fact that ME-180cells expressing sLZIP siRNA were refractory to MMP-9 expression. Results from wound healing and invasion assays showed that sLZIP enhanced both the migration and invasion of cervical cancer cells. The increased migration and invasion of HeLa and SiHa cells that were induced by sLZIP were abrogated by inhibition of the proteolytic activity of MMP-9.These results indicate that sLZIP plays a critical role in MMP-9expression and is probably involved in invasion and metastasis of cervical cancer.

Original languageEnglish
Pages (from-to)42072-42081
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number49
DOIs
Publication statusPublished - 2011 Dec 9

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Leucine Zippers
Matrix Metalloproteinase 9
Uterine Cervical Neoplasms
Cells
Matrix Metalloproteinases
Proteolysis
Proteins
HeLa Cells
Tissue homeostasis
Transcription Factors
Neoplasm Metastasis
Inflammation
Glucocorticoid Receptors
Refractory materials
Wound Healing
Small Interfering RNA
Transcriptional Activation
Tumors
Assays
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Human leucine zipper protein sLZIP induces migration and invasion of cervical cancer cells via expression of matrix metalloproteinase-9. / Kang, Hyereen; Jang, Sung Wuk; Ko, Je Sang.

In: Journal of Biological Chemistry, Vol. 286, No. 49, 09.12.2011, p. 42072-42081.

Research output: Contribution to journalArticle

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