Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain

Nayoung Kim, Jae Cheal Yoo, Jae Yoon Han, Eun Mi Hwang, Yoon Sook Kim, Eun Young Jeong, Choong Hyun Sun, Gwan Su Yi, Gu Seob Roh, Hyun Joon Kim, Sang Soo Kang, Gyeong Jae Cho, Jae-Yong Park, Wan Sung Choi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Clusterin (CLU), a glycoprotein, is involved in apoptosis, producing two alternatively spliced isoforms in various cell types. The pro-apoptotic CLU appears to be a nuclear isoform (nuclear clusterin; nCLU), and the secretory CLU (sCLU) is thought to be anti-apoptotic. The detailed molecular mechanism of nCLU as a pro-apoptotic molecule has not yet been clear. In the current study, overexpressed nCLU induced apoptosis in human kidney cells. Biochemical studies revealed that nCLU sequestered Bcl-XL via a putative BH3 motif in the C-terminal coiled coil (CC2) domain, releasing Bax, and promoted apoptosis accompanied by activation of caspase-3 and cytochrome c release. These results suggest a novel mechanism of apoptosis mediated by nCLU as a pro-apoptotic molecule.

Original languageEnglish
Pages (from-to)1157-1167
Number of pages11
JournalJournal of Cellular Physiology
Volume227
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1
Externally publishedYes

Fingerprint

Clusterin
Apoptosis
Protein Isoforms
Molecules
Cytochromes c
Caspase 3
Glycoproteins
Chemical activation
Kidney

Keywords

  • Apoptosis
  • Bcl-XL
  • Clusterin

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kim, N., Yoo, J. C., Han, J. Y., Hwang, E. M., Kim, Y. S., Jeong, E. Y., ... Choi, W. S. (2012). Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain. Journal of Cellular Physiology, 227(3), 1157-1167. https://doi.org/10.1002/jcp.22836

Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain. / Kim, Nayoung; Yoo, Jae Cheal; Han, Jae Yoon; Hwang, Eun Mi; Kim, Yoon Sook; Jeong, Eun Young; Sun, Choong Hyun; Yi, Gwan Su; Roh, Gu Seob; Kim, Hyun Joon; Kang, Sang Soo; Cho, Gyeong Jae; Park, Jae-Yong; Choi, Wan Sung.

In: Journal of Cellular Physiology, Vol. 227, No. 3, 01.03.2012, p. 1157-1167.

Research output: Contribution to journalArticle

Kim, N, Yoo, JC, Han, JY, Hwang, EM, Kim, YS, Jeong, EY, Sun, CH, Yi, GS, Roh, GS, Kim, HJ, Kang, SS, Cho, GJ, Park, J-Y & Choi, WS 2012, 'Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain', Journal of Cellular Physiology, vol. 227, no. 3, pp. 1157-1167. https://doi.org/10.1002/jcp.22836
Kim, Nayoung ; Yoo, Jae Cheal ; Han, Jae Yoon ; Hwang, Eun Mi ; Kim, Yoon Sook ; Jeong, Eun Young ; Sun, Choong Hyun ; Yi, Gwan Su ; Roh, Gu Seob ; Kim, Hyun Joon ; Kang, Sang Soo ; Cho, Gyeong Jae ; Park, Jae-Yong ; Choi, Wan Sung. / Human nuclear clusterin mediates apoptosis by interacting with Bcl-XL through C-terminal coiled coil domain. In: Journal of Cellular Physiology. 2012 ; Vol. 227, No. 3. pp. 1157-1167.
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