Human papillomavirus genotyping by oligonucleotide microarray and p16 INK4A expression in uterine cervical intraepithelial neoplasm and in invasive carcinoma in Korean women

Zhen Hua Lin, Xiong Hu Shen, Zhe Jin, Young Sik Kim, Eung Seok Lee, Han Kyeom Kim, Insun Kim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

For evaluating the diagnostic significance of p16INK4A overexpression in the uterine cervical intraepithelial neoplasm and in invasive carcinoma, human papillomavirus (HPV) was detected and genotyped by oligonucleotide microarray in archival tissues of 117 cervical specimens, including 47 invasive squamous cell carcinomas (SCC), 30 cases of cervical intraepithelial neoplasia (CIN), 20 adenocarcinomas, and 20 cases of non-neoplastic cervix. The expression of p16INK4A protein was immunohistochemically studied in these cases and in five HPV-positive and one HPV-negative cervical cancer cell lines. HPV was detected in 50% of CIN, 61.7% of SCC, and 45.5% of adenocarcinomas. p16INK4A expression was seen in all 20 cases of adenocarcinoma, 78.7% (37/47) of SCC, and 96.7% (29/30) of CIN, but not in any cases of the non-neoplastic cervix. There was no difference in p16INK4A expression between the HPV-positive and HPV-negative cervical lesions. All HPV-positive and -negative cervical cancer cell lines expressed p16INK4A protein. In conclusion, the presence of p16 INK4A expression in cervical squamous and glandular epithelium indicates the existence of dysplasia or malignancy in the uterine cervix, regardless of HPV infection.

Original languageEnglish
Pages (from-to)491-496
Number of pages6
JournalPathology International
Volume55
Issue number8
DOIs
Publication statusPublished - 2005 Aug 1

Fingerprint

Cyclin-Dependent Kinase Inhibitor p16
Cervical Intraepithelial Neoplasia
Oligonucleotide Array Sequence Analysis
Uterine Cervical Neoplasms
Carcinoma
Cervix Uteri
Squamous Cell Carcinoma
Adenocarcinoma
Cell Line
Papillomavirus Infections
Epithelium

Keywords

  • Cervix uteri
  • Genotype
  • Human papillomavirus
  • Oligonucleotide microarray
  • p16

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

@article{277dc35dd2324120ada604eb01102218,
title = "Human papillomavirus genotyping by oligonucleotide microarray and p16 INK4A expression in uterine cervical intraepithelial neoplasm and in invasive carcinoma in Korean women",
abstract = "For evaluating the diagnostic significance of p16INK4A overexpression in the uterine cervical intraepithelial neoplasm and in invasive carcinoma, human papillomavirus (HPV) was detected and genotyped by oligonucleotide microarray in archival tissues of 117 cervical specimens, including 47 invasive squamous cell carcinomas (SCC), 30 cases of cervical intraepithelial neoplasia (CIN), 20 adenocarcinomas, and 20 cases of non-neoplastic cervix. The expression of p16INK4A protein was immunohistochemically studied in these cases and in five HPV-positive and one HPV-negative cervical cancer cell lines. HPV was detected in 50{\%} of CIN, 61.7{\%} of SCC, and 45.5{\%} of adenocarcinomas. p16INK4A expression was seen in all 20 cases of adenocarcinoma, 78.7{\%} (37/47) of SCC, and 96.7{\%} (29/30) of CIN, but not in any cases of the non-neoplastic cervix. There was no difference in p16INK4A expression between the HPV-positive and HPV-negative cervical lesions. All HPV-positive and -negative cervical cancer cell lines expressed p16INK4A protein. In conclusion, the presence of p16 INK4A expression in cervical squamous and glandular epithelium indicates the existence of dysplasia or malignancy in the uterine cervix, regardless of HPV infection.",
keywords = "Cervix uteri, Genotype, Human papillomavirus, Oligonucleotide microarray, p16",
author = "Lin, {Zhen Hua} and Shen, {Xiong Hu} and Zhe Jin and Kim, {Young Sik} and Lee, {Eung Seok} and Kim, {Han Kyeom} and Insun Kim",
year = "2005",
month = "8",
day = "1",
doi = "10.1111/j.1440-1827.2005.01858.x",
language = "English",
volume = "55",
pages = "491--496",
journal = "Pathology International",
issn = "1320-5463",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Human papillomavirus genotyping by oligonucleotide microarray and p16 INK4A expression in uterine cervical intraepithelial neoplasm and in invasive carcinoma in Korean women

AU - Lin, Zhen Hua

AU - Shen, Xiong Hu

AU - Jin, Zhe

AU - Kim, Young Sik

AU - Lee, Eung Seok

AU - Kim, Han Kyeom

AU - Kim, Insun

PY - 2005/8/1

Y1 - 2005/8/1

N2 - For evaluating the diagnostic significance of p16INK4A overexpression in the uterine cervical intraepithelial neoplasm and in invasive carcinoma, human papillomavirus (HPV) was detected and genotyped by oligonucleotide microarray in archival tissues of 117 cervical specimens, including 47 invasive squamous cell carcinomas (SCC), 30 cases of cervical intraepithelial neoplasia (CIN), 20 adenocarcinomas, and 20 cases of non-neoplastic cervix. The expression of p16INK4A protein was immunohistochemically studied in these cases and in five HPV-positive and one HPV-negative cervical cancer cell lines. HPV was detected in 50% of CIN, 61.7% of SCC, and 45.5% of adenocarcinomas. p16INK4A expression was seen in all 20 cases of adenocarcinoma, 78.7% (37/47) of SCC, and 96.7% (29/30) of CIN, but not in any cases of the non-neoplastic cervix. There was no difference in p16INK4A expression between the HPV-positive and HPV-negative cervical lesions. All HPV-positive and -negative cervical cancer cell lines expressed p16INK4A protein. In conclusion, the presence of p16 INK4A expression in cervical squamous and glandular epithelium indicates the existence of dysplasia or malignancy in the uterine cervix, regardless of HPV infection.

AB - For evaluating the diagnostic significance of p16INK4A overexpression in the uterine cervical intraepithelial neoplasm and in invasive carcinoma, human papillomavirus (HPV) was detected and genotyped by oligonucleotide microarray in archival tissues of 117 cervical specimens, including 47 invasive squamous cell carcinomas (SCC), 30 cases of cervical intraepithelial neoplasia (CIN), 20 adenocarcinomas, and 20 cases of non-neoplastic cervix. The expression of p16INK4A protein was immunohistochemically studied in these cases and in five HPV-positive and one HPV-negative cervical cancer cell lines. HPV was detected in 50% of CIN, 61.7% of SCC, and 45.5% of adenocarcinomas. p16INK4A expression was seen in all 20 cases of adenocarcinoma, 78.7% (37/47) of SCC, and 96.7% (29/30) of CIN, but not in any cases of the non-neoplastic cervix. There was no difference in p16INK4A expression between the HPV-positive and HPV-negative cervical lesions. All HPV-positive and -negative cervical cancer cell lines expressed p16INK4A protein. In conclusion, the presence of p16 INK4A expression in cervical squamous and glandular epithelium indicates the existence of dysplasia or malignancy in the uterine cervix, regardless of HPV infection.

KW - Cervix uteri

KW - Genotype

KW - Human papillomavirus

KW - Oligonucleotide microarray

KW - p16

UR - http://www.scopus.com/inward/record.url?scp=23244456243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23244456243&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1827.2005.01858.x

DO - 10.1111/j.1440-1827.2005.01858.x

M3 - Article

VL - 55

SP - 491

EP - 496

JO - Pathology International

JF - Pathology International

SN - 1320-5463

IS - 8

ER -