TY - JOUR
T1 - Human papillomavirus is more frequently detected in the pelvic than non-pelvic area in patients with squamous cell carcinoma in situ (Bowen’s disease)
AU - Baek, Yoo Sang
AU - Jeon, Jiehyun
AU - Kim, Aeree
AU - Song, Hae Jun
AU - Kim, Chungyeul
N1 - Funding Information:
Funding sources: This study was supported by a Korea University Research Grant and by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF- 2018R1D1A1B07045088). Conflicts of interest: none.
Publisher Copyright:
© 2020, JLE/Springer.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - Background: The detection rate of human papillomavirus (HPV) in Bowen’s disease (BD) varies greatly. Objectives: To detect HPV DNA in BD samples using next-generation sequencing (NGS) and compare HPV detection rates between pelvic and non-pelvic BD. Materials and Methods: We evaluated 99 patients with BD in our institution. DNA was extracted from formalin-fixed and paraffin embedded (FFPE) tissue blocks. The presence of HPV DNA material was detected using special kit-based NGS technology. Clinical characteristics and HPV detection rates were then compared between pelvic and non-pelvic BD samples. Results: HPV was detected in 26 (26.3%) BD samples. A total of 10 types of α-HPV was detected: HPV 16, 53, 31, 58, 66, 26, 27, 57, 45, and 72. The most common HPV type was 16 (12.1%). Only two types (27 and 57) were frequently classified as cutaneous type, and the rest were mucosal types. The HPV detection rate was significantly higher in pelvic BD (45.2%) compared to non-pelvic BD (17.6%). Conclusion: The present study suggests that sexually transmitted mucosal α-HPV plays a significant role in the pathogenesis of BD, especially in the pelvic region.
AB - Background: The detection rate of human papillomavirus (HPV) in Bowen’s disease (BD) varies greatly. Objectives: To detect HPV DNA in BD samples using next-generation sequencing (NGS) and compare HPV detection rates between pelvic and non-pelvic BD. Materials and Methods: We evaluated 99 patients with BD in our institution. DNA was extracted from formalin-fixed and paraffin embedded (FFPE) tissue blocks. The presence of HPV DNA material was detected using special kit-based NGS technology. Clinical characteristics and HPV detection rates were then compared between pelvic and non-pelvic BD samples. Results: HPV was detected in 26 (26.3%) BD samples. A total of 10 types of α-HPV was detected: HPV 16, 53, 31, 58, 66, 26, 27, 57, 45, and 72. The most common HPV type was 16 (12.1%). Only two types (27 and 57) were frequently classified as cutaneous type, and the rest were mucosal types. The HPV detection rate was significantly higher in pelvic BD (45.2%) compared to non-pelvic BD (17.6%). Conclusion: The present study suggests that sexually transmitted mucosal α-HPV plays a significant role in the pathogenesis of BD, especially in the pelvic region.
KW - Bowen’s disease
KW - Human papillomavirus
KW - Next-generation sequencing
KW - Squamous cell carcinoma in situ
KW - α-human papillomavirus
UR - http://www.scopus.com/inward/record.url?scp=85086555889&partnerID=8YFLogxK
U2 - 10.1684/ejd.2020.3700
DO - 10.1684/ejd.2020.3700
M3 - Article
C2 - 32538355
AN - SCOPUS:85086555889
VL - 30
SP - 111
EP - 118
JO - European Journal of Dermatology
JF - European Journal of Dermatology
SN - 1167-1122
IS - 2
ER -