Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death

Soo Hyun Choi, So Young Kim, Jae Jin An, Sun Hwa Lee, Dae Won Kim, Hea Jin Ryu, Nam Il Lee, Seung Il Yeo, Sang Ho Jang, Moo Ho Won, Tae Cheon Kang, Hyung Joo Kwon, Sung Woo Cho, Joon Kim, Kil Soo Lee, Jinseu Park, Won Sik Eum, Soo Young Choi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The consequences of ultraviolet (UV) exposure are implicated in skin aging and cell death. The ribosomal protein S3 (rpS3) is one of the major proteins by which cells counteract the deleterious effects of UV and it plays a role in the repair of damaged DNA. In the present study, we investigated the protective effects of PEP-1-rpS3 fusion protein after UV-induced cell injury. A human rpS3 gene was fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-rpS3 fusion protein. The expressed and purified fusion proteins were efficiently transduced into skin cells in a time- and dose-dependent manner. Once inside the cells, transduced PEP-1-rpS3 fusion protein was stable for 48 h. We showed that transduced PEP-1-rpS3 fusion protein increased cell viability and dramatically reduced DNA lesions in the UV exposed skin cells. Immunohistochemical analysis revealed that PEP-1-rpS3 fusion protein efficiently penetrated the epidermis as well as the dermis of the subcutaneous layer when sprayed on animal skin. These results suggest that PEP-1-rpS3 fusion protein can be used in protein therapy for various disorders related to UV, including skin aging and cancer.

Original languageEnglish
Pages (from-to)6755-6762
Number of pages8
JournalFEBS Letters
Volume580
Issue number30
DOIs
Publication statusPublished - 2006 Dec 22

Keywords

  • PEP-1 peptide
  • Protein therapy
  • Protein transduction
  • Ribosomal protein S3
  • Ultraviolet-damage

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Choi, S. H., Kim, S. Y., An, J. J., Lee, S. H., Kim, D. W., Ryu, H. J., Lee, N. I., Yeo, S. I., Jang, S. H., Won, M. H., Kang, T. C., Kwon, H. J., Cho, S. W., Kim, J., Lee, K. S., Park, J., Eum, W. S., & Choi, S. Y. (2006). Human PEP-1-ribosomal protein S3 protects against UV-induced skin cell death. FEBS Letters, 580(30), 6755-6762. https://doi.org/10.1016/j.febslet.2006.11.038