Abstract
Co-culture of human embryonic stem (ES) cells on mouse fibroblast feeders is the commonly used method for in vitro expansion of human ES cells in an undifferentiated state. However, it has potential risks of pathogen transmission from animals; thus, human cell-derived feeders have been employed to minimize this problem. In this study, we compared human placenta-derived feeders with bone marrow to demonstrate its effectiveness as feeders for in vitro long-term culture of human ES cells. We cultured a human ES cell line, SNUhES3, on human placenta-derived mesenchymal stem cell feeders and compared their culture efficiency with human bone marrow-derived feeders and control group (mouse fibroblast feeders, STO). The mean number of human ES cell colonies was 166 ± 35 in the placenta feeders; this was significantly higher than bone marrow-derived feeders (87 ± 16, p < 0.05). We could propagate the culture of SNUhES3 on the placenta feeders past the 50th week similar to control group. During the culture, the maintenance of undifferentiated state of SNUhES3 was demonstrated by the expression of SSEA-4, TRA-1-81, TRA-1-60, and Oct-4. However, we failed to propagate the culture of human ES cells on the human bone marrow-derived feeders past the 5th week. The efficiency of embryoid body formation was similar between placenta and control group, indicating the preservation of differentiation ability. Thus, placenta-derived feeders are more efficient for the long-term in vitro culture of human ES cells than bone marrow-derived feeders suggesting the possible role of placenta as a source for human cell-derived feeders.
| Original language | English |
|---|---|
| Pages (from-to) | 421-428 |
| Number of pages | 8 |
| Journal | Stem Cells and Development |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2007 Jun 1 |
Fingerprint
ASJC Scopus subject areas
- Hematology
Cite this
Human placenta-derived feeders support prolonged undifferentiated propagation of a human embryonic stem cell line, SNUhES3 : Comparison with human bone marrow-derived feeders. / Seok, Jin Kim; Chang, Hee Song; Sung, Hwa Jung; Yoo, Young Do; Geum, Dongho; Sun, Hwa Park; Ji, Hyun Yoo; Jee, Hyun Oh; Hye, Jin Shin; Sun, Haeng Kim; Kim, Jun Suk; Kim, Byung Soo.
In: Stem Cells and Development, Vol. 16, No. 3, 01.06.2007, p. 421-428.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human placenta-derived feeders support prolonged undifferentiated propagation of a human embryonic stem cell line, SNUhES3
T2 - Comparison with human bone marrow-derived feeders
AU - Seok, Jin Kim
AU - Chang, Hee Song
AU - Sung, Hwa Jung
AU - Yoo, Young Do
AU - Geum, Dongho
AU - Sun, Hwa Park
AU - Ji, Hyun Yoo
AU - Jee, Hyun Oh
AU - Hye, Jin Shin
AU - Sun, Haeng Kim
AU - Kim, Jun Suk
AU - Kim, Byung Soo
PY - 2007/6/1
Y1 - 2007/6/1
N2 - Co-culture of human embryonic stem (ES) cells on mouse fibroblast feeders is the commonly used method for in vitro expansion of human ES cells in an undifferentiated state. However, it has potential risks of pathogen transmission from animals; thus, human cell-derived feeders have been employed to minimize this problem. In this study, we compared human placenta-derived feeders with bone marrow to demonstrate its effectiveness as feeders for in vitro long-term culture of human ES cells. We cultured a human ES cell line, SNUhES3, on human placenta-derived mesenchymal stem cell feeders and compared their culture efficiency with human bone marrow-derived feeders and control group (mouse fibroblast feeders, STO). The mean number of human ES cell colonies was 166 ± 35 in the placenta feeders; this was significantly higher than bone marrow-derived feeders (87 ± 16, p < 0.05). We could propagate the culture of SNUhES3 on the placenta feeders past the 50th week similar to control group. During the culture, the maintenance of undifferentiated state of SNUhES3 was demonstrated by the expression of SSEA-4, TRA-1-81, TRA-1-60, and Oct-4. However, we failed to propagate the culture of human ES cells on the human bone marrow-derived feeders past the 5th week. The efficiency of embryoid body formation was similar between placenta and control group, indicating the preservation of differentiation ability. Thus, placenta-derived feeders are more efficient for the long-term in vitro culture of human ES cells than bone marrow-derived feeders suggesting the possible role of placenta as a source for human cell-derived feeders.
AB - Co-culture of human embryonic stem (ES) cells on mouse fibroblast feeders is the commonly used method for in vitro expansion of human ES cells in an undifferentiated state. However, it has potential risks of pathogen transmission from animals; thus, human cell-derived feeders have been employed to minimize this problem. In this study, we compared human placenta-derived feeders with bone marrow to demonstrate its effectiveness as feeders for in vitro long-term culture of human ES cells. We cultured a human ES cell line, SNUhES3, on human placenta-derived mesenchymal stem cell feeders and compared their culture efficiency with human bone marrow-derived feeders and control group (mouse fibroblast feeders, STO). The mean number of human ES cell colonies was 166 ± 35 in the placenta feeders; this was significantly higher than bone marrow-derived feeders (87 ± 16, p < 0.05). We could propagate the culture of SNUhES3 on the placenta feeders past the 50th week similar to control group. During the culture, the maintenance of undifferentiated state of SNUhES3 was demonstrated by the expression of SSEA-4, TRA-1-81, TRA-1-60, and Oct-4. However, we failed to propagate the culture of human ES cells on the human bone marrow-derived feeders past the 5th week. The efficiency of embryoid body formation was similar between placenta and control group, indicating the preservation of differentiation ability. Thus, placenta-derived feeders are more efficient for the long-term in vitro culture of human ES cells than bone marrow-derived feeders suggesting the possible role of placenta as a source for human cell-derived feeders.
UR - http://www.scopus.com/inward/record.url?scp=34347346069&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34347346069&partnerID=8YFLogxK
U2 - 10.1089/scd.2006.0098
DO - 10.1089/scd.2006.0098
M3 - Article
C2 - 17610372
AN - SCOPUS:34347346069
VL - 16
SP - 421
EP - 428
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 3
ER -