HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions

Incheol Ryu, Yeonkyoung Park, Jwa Won Seo, Ok Hyun Park, Hongseok Ha, Jin Wu Nam, Yoon Ki Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

All metazoan mRNAs have a poly(A) tail at the 3' end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 3' UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.-Ryu, I., Park, Y., Seo, J.-W., Park, O. H., Ha, H., Nam, J.-W., Kim, Y. K. HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions.

Original languageEnglish
Pages (from-to)2680-2693
Number of pages14
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number2
DOIs
Publication statusPublished - 2019 Feb 1

Fingerprint

Histones
Antigens
Messenger RNA
Poly A
Puromycin
3' Untranslated Regions
Stabilization
Genes
Irradiation
Genome

Keywords

  • 3′UTR
  • mRNA stability
  • polyadenylation signal
  • UVC

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions. / Ryu, Incheol; Park, Yeonkyoung; Seo, Jwa Won; Park, Ok Hyun; Ha, Hongseok; Nam, Jin Wu; Kim, Yoon Ki.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 2, 01.02.2019, p. 2680-2693.

Research output: Contribution to journalArticle

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