HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions

Incheol Ryu, Yeonkyoung Park, Jwa Won Seo, Ok Hyun Park, Hongseok Ha, Jin Wu Nam, Yoon Ki Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.

Original languageEnglish
Pages (from-to)2680-2693
Number of pages14
JournalFASEB Journal
Volume33
Issue number2
DOIs
Publication statusPublished - 2019

Keywords

  • 39UTR
  • Polyadenylation signal
  • UVC
  • mRNA stability

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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