HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions

Incheol Ryu; Yeonkyoung Park; Jwa Won Seo; Ok Hyun Park; Hongseok Ha; Jin Wu Nam; Yoon Ki Kim

doi:10.1096/fj.201800431R

HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions

Incheol Ryu, Yeonkyoung Park, Jwa Won Seo, Ok Hyun Park, Hongseok Ha, Jin Wu Nam, Yoon Ki Kim

Division of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)⁺ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)⁺ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)⁺ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)⁺ RDH mRNAs.

Original language	English
Pages (from-to)	2680-2693
Number of pages	14
Journal	FASEB Journal
Volume	33
Issue number	2
DOIs	https://doi.org/10.1096/fj.201800431R
Publication status	Published - 2019

Keywords

39UTR
Polyadenylation signal
UVC
mRNA stability

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Genetics

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title = "HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions",

abstract = "All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.",

keywords = "39UTR, Polyadenylation signal, UVC, mRNA stability",

author = "Incheol Ryu and Yeonkyoung Park and Seo, {Jwa Won} and Park, {Ok Hyun} and Hongseok Ha and Nam, {Jin Wu} and Kim, {Yoon Ki}",

note = "Funding Information: The authors thank Dr. Berndt M{\"u}ller (University of Aberdeen, Aberdeen, United Kingdom) for providing the antibody against human SLBP, Dr. Lynne E. Maquat (University of Rochester, Rochester, NY, USA) for the antibody against human UPF1, and Dr. You-Sub Won (Korea University) for scientific comments. This work was supported by the National Research Foundation (NRF) of Korea funded by the Korean government (MSIP) (Grants NRF-2015R1A3A2033665 and NRF-2018R1A5A1024261 to Y.K.K.; and, in part, 2013R1A1A2063273 and 2016R1D1A1B3933894 to I.R.), and by a Korea University grant (to Y.K.K.). The authors declare no conflicts of interest.",

year = "2019",

doi = "10.1096/fj.201800431R",

language = "English",

volume = "33",

pages = "2680--2693",

journal = "The FASEB journal : official publication of the Federation of American Societies for Experimental Biology",

issn = "1530-6860",

publisher = "FASEB",

number = "2",

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TY - JOUR

T1 - HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions

AU - Ryu, Incheol

AU - Park, Yeonkyoung

AU - Seo, Jwa Won

AU - Park, Ok Hyun

AU - Ha, Hongseok

AU - Nam, Jin Wu

AU - Kim, Yoon Ki

N1 - Funding Information: The authors thank Dr. Berndt Müller (University of Aberdeen, Aberdeen, United Kingdom) for providing the antibody against human SLBP, Dr. Lynne E. Maquat (University of Rochester, Rochester, NY, USA) for the antibody against human UPF1, and Dr. You-Sub Won (Korea University) for scientific comments. This work was supported by the National Research Foundation (NRF) of Korea funded by the Korean government (MSIP) (Grants NRF-2015R1A3A2033665 and NRF-2018R1A5A1024261 to Y.K.K.; and, in part, 2013R1A1A2063273 and 2016R1D1A1B3933894 to I.R.), and by a Korea University grant (to Y.K.K.). The authors declare no conflicts of interest.

PY - 2019

Y1 - 2019

N2 - All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.

AB - All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.

KW - 39UTR

KW - Polyadenylation signal

KW - UVC

KW - mRNA stability

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JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

SN - 1530-6860

IS - 2

ER -

HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions

Abstract

Keywords

ASJC Scopus subject areas

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