Abstract
All metazoan mRNAs have a poly(A) tail at the 39 end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 39 UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.
Original language | English |
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Pages (from-to) | 2680-2693 |
Number of pages | 14 |
Journal | FASEB Journal |
Volume | 33 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2019 |
Keywords
- 39UTR
- Polyadenylation signal
- UVC
- mRNA stability
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Genetics