Abstract
All metazoan mRNAs have a poly(A) tail at the 3' end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 3' UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.-Ryu, I., Park, Y., Seo, J.-W., Park, O. H., Ha, H., Nam, J.-W., Kim, Y. K. HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 2680-2693 |
| Number of pages | 14 |
| Journal | FASEB journal : official publication of the Federation of American Societies for Experimental Biology |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2019 Feb 1 |
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Keywords
- 3′UTR
- mRNA stability
- polyadenylation signal
- UVC
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Genetics
Cite this
HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions. / Ryu, Incheol; Park, Yeonkyoung; Seo, Jwa Won; Park, Ok Hyun; Ha, Hongseok; Nam, Jin Wu; Kim, Yoon Ki.
In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 2, 01.02.2019, p. 2680-2693.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions
AU - Ryu, Incheol
AU - Park, Yeonkyoung
AU - Seo, Jwa Won
AU - Park, Ok Hyun
AU - Ha, Hongseok
AU - Nam, Jin Wu
AU - Kim, Yoon Ki
PY - 2019/2/1
Y1 - 2019/2/1
N2 - All metazoan mRNAs have a poly(A) tail at the 3' end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 3' UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.-Ryu, I., Park, Y., Seo, J.-W., Park, O. H., Ha, H., Nam, J.-W., Kim, Y. K. HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions.
AB - All metazoan mRNAs have a poly(A) tail at the 3' end with the exception of replication-dependent histone (RDH) mRNAs, which end in a highly conserved stem-loop (SL) structure. However, a subset of RDH mRNAs are reported to be polyadenylated under physiologic conditions. The molecular details of the biogenesis of polyadenylated RDH [poly(A)+ RDH] mRNAs remain unknown. In this study, our genome-wide analyses reveal that puromycin treatment or UVC irradiation stabilizes poly(A)+ RDH mRNAs, relative to canonical RDH mRNAs, which end in an SL structure. We demonstrate that the stabilization of poly(A)+ RDH mRNAs occurs in a translation-independent manner and is regulated via human antigen R (HuR) binding to the extended 3' UTR under stress conditions. Our data suggest that HuR regulates the expression of poly(A)+ RDH mRNAs.-Ryu, I., Park, Y., Seo, J.-W., Park, O. H., Ha, H., Nam, J.-W., Kim, Y. K. HuR stabilizes a polyadenylated form of replication-dependent histone mRNAs under stress conditions.
KW - 3′UTR
KW - mRNA stability
KW - polyadenylation signal
KW - UVC
UR - http://www.scopus.com/inward/record.url?scp=85061059167&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061059167&partnerID=8YFLogxK
U2 - 10.1096/fj.201800431R
DO - 10.1096/fj.201800431R
M3 - Article
VL - 33
SP - 2680
EP - 2693
JO - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
JF - The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
SN - 1530-6860
IS - 2
ER -