Hyaline cartilage regeneration by combined therapy of microfracture and long-term bone morphogenetic protein-2 delivery

Hee Seok Yang, Wan Geun La, Suk Ho Bhang, Hak Jun Kim, Gun Il Im, Haeshin Lee, Jung-Ho Park, Byung Soo Kim

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Microfracture of cartilage induces migration of bone-marrow-derived mesenchymal stem cells. However, this treatment often results in fibrocartilage regeneration. Growth factors such as bone morphogenetic protein (BMP)-2 induce the differentiation of bone-marrow-derived mesenchymal stem cells into chondrocytes, which can be used for hyaline cartilage regeneration. Here, we tested the hypothesis that long-term delivery of BMP-2 to cartilage defects subjected to microfracture results in regeneration of high-quality hyaline-like cartilage, as opposed to short-term delivery of BMP-2 or no BMP-2 delivery. Heparin-conjugated fibrin (HCF) and normal fibrin were used as carriers for the long- and short-term delivery of BMP-2, respectively. Rabbit articular cartilage defects were treated with microfracture combined with one of the following: no treatment, fibrin, short-term delivery of BMP-2, HCF, or long-term delivery of BMP-2. Eight weeks after treatment, histological analysis revealed that the long-term delivery of BMP-2 group (microfracture + HCF + BMP-2) showed the most staining with alcian blue. A biochemical assay, real-time polymerase chain reaction assay and Western blot analysis all revealed that the long-term delivery of BMP-2 group had the highest glucosaminoglycan content as well as the highest expression level of collagen type II. Taken together, the long-term delivery of BMP-2 to cartilage defects subjected to microfracture resulted in regeneration of hyaline-like cartilage, as opposed to short-term delivery or no BMP-2 delivery. Therefore, this method could be more convenient for hyaline cartilage regeneration than autologous chondrocyte implantation due to its less invasive nature and lack of cell implantation.

Original languageEnglish
Pages (from-to)1809-1818
Number of pages10
JournalTissue Engineering - Part A
Volume17
Issue number13-14
DOIs
Publication statusPublished - 2011 Jul 1

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Hyaline Cartilage
Bone Morphogenetic Protein 2
Stress Fractures
Cartilage
Regeneration
Bone
Proteins
Fibrin
Therapeutics
Heparin
Chondrocytes
Stem cells
Mesenchymal Stromal Cells
Defects
Assays
Bone Marrow
Fibrocartilage
Alcian Blue
Collagen Type II
Polymerase chain reaction

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Hyaline cartilage regeneration by combined therapy of microfracture and long-term bone morphogenetic protein-2 delivery. / Yang, Hee Seok; La, Wan Geun; Bhang, Suk Ho; Kim, Hak Jun; Im, Gun Il; Lee, Haeshin; Park, Jung-Ho; Kim, Byung Soo.

In: Tissue Engineering - Part A, Vol. 17, No. 13-14, 01.07.2011, p. 1809-1818.

Research output: Contribution to journalArticle

Yang, Hee Seok ; La, Wan Geun ; Bhang, Suk Ho ; Kim, Hak Jun ; Im, Gun Il ; Lee, Haeshin ; Park, Jung-Ho ; Kim, Byung Soo. / Hyaline cartilage regeneration by combined therapy of microfracture and long-term bone morphogenetic protein-2 delivery. In: Tissue Engineering - Part A. 2011 ; Vol. 17, No. 13-14. pp. 1809-1818.
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