Hyaluronic acid-ceramide-based optical/MR dual imaging nanoprobe for cancer diagnosis

Hyun Jong Cho, Hong Yeol Yoon, Heebeom Koo, Seung Hak Ko, Jae Seong Shim, Jee Hyun Cho, Jae Hyung Park, Kwang Meyung Kim, Ick Chan Kwon, Dae Duk Kim

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61 Citations (Scopus)

Abstract

Hyaluronic acid-ceramide (HACE)-based nanoprobes for magnetic resonance (MR) and optical imaging were developed for cancer diagnosis. Diethylenetriaminepentaacetic dianhydride (DTPA) was conjugated to HACE for the chelation of gadolinium (Gd) as an MR contrast agent. Cy5.5 was also conjugated to the HACE backbone as a near-infrared fluorescence (NIRF) imaging dye. The self-assembled HACE-based nanoprobe, Cy5.5-HACE-DTPA-Gd, exhibited a uniformly distributed particle size and morphological shape. The HACE-based nanoprobe did not induce serious cytotoxicity in U87-MG (low expression of CD44 receptor) and SCC7 (high expression of CD44 receptor) cells. The cellular uptake efficiency of the HACE-based nanoprobe was higher in SCC7 cells than in U87-MG cells, indicating an HA-CD44 receptor interaction. In vitro MR signal enhancement of the HACE-based nanoprobe was confirmed compared with a commercial formulation (Magnevist). Moreover, in vivo MR contrast enhancement of the HACE-based nanoprobe in the tumor region was verified in an SCC7 tumor xenograft mouse model. The tumor targetability of the developed nanoprobe was monitored by an NIRF imaging study, and improved accumulation of the nanoprobe in the tumor region was observed. Therefore, this HACE-based dual-imaging nanoprobe can be used to make a more accurate diagnosis of cancer based on its passive and active tumor targeting strategies.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalJournal of Controlled Release
Volume162
Issue number1
DOIs
Publication statusPublished - 2012 Aug 20
Externally publishedYes

Keywords

  • Dual modality
  • Hyaluronic acid-ceramide
  • MRI
  • NIRF
  • Tumor targeting

ASJC Scopus subject areas

  • Pharmaceutical Science

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  • Cite this

    Cho, H. J., Yoon, H. Y., Koo, H., Ko, S. H., Shim, J. S., Cho, J. H., Park, J. H., Kim, K. M., Kwon, I. C., & Kim, D. D. (2012). Hyaluronic acid-ceramide-based optical/MR dual imaging nanoprobe for cancer diagnosis. Journal of Controlled Release, 162(1), 111-118. https://doi.org/10.1016/j.jconrel.2012.06.011