Hyaluronic acid derived from other streptococci supports streptococcus pneumoniae in vitro biofilm formation

Mukesh Kumar Yadav, Sungwon Chae, Kyeongsoon Park, Jae-Jun Song

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigate the role of hyaluronic acid (HA) on S. pneumoniae in vitro biofilm formation and evaluate gene expressions of virulence and/or biofilm related genes. Biofilms were grown in medium supplied with HA derived from capsule of Streptococcus equi. The biomasses of biofilms were detected by crystal-violet (CV) microtiter plate assay, and the morphology was viewed under scanning electron microscope (SEM). The gene expressions were assessed by relative quantitative RT-PCR. The results showed that the HA support pneumococcal growth in planktonic form and within biofilms. The CV-microtiter plate assay detected significantly increased biofilm growth in medium containing HA. The SEM analysis revealed thick and organized biofilms in positive control and HA supplemented medium. The nanA, nanB, bgaA, strH, luxS, hysA, ugl, and PST-EIIA encoding genes were significantly upregulated in the planktonic cells grown in presence of HA, while the lytA and comA genes were downregulated. Similarly the luxS, hysA, ugl, and PST-EIIA encoding genes were significantly upregulated by more than 2-folds in HA biofilms. The results of this study indicate that the HA derived from capsule of S. equi supports pneumococcal growth in planktonic state and within biofilms and upregulated virulence and biofilm related genes.

Original languageEnglish
Article number690217
JournalBioMed Research International
Volume2013
DOIs
Publication statusPublished - 2013 Oct 21

Fingerprint

Biofilms
Hyaluronic Acid
Streptococcus pneumoniae
Streptococcus
Streptococcus equi
Gentian Violet
Gene encoding
Genes
Gene expression
Capsules
Virulence
Assays
Electron microscopes
Growth
In Vitro Techniques
Electrons
Scanning
Gene Expression
Coma
Biomass

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Hyaluronic acid derived from other streptococci supports streptococcus pneumoniae in vitro biofilm formation. / Yadav, Mukesh Kumar; Chae, Sungwon; Park, Kyeongsoon; Song, Jae-Jun.

In: BioMed Research International, Vol. 2013, 690217, 21.10.2013.

Research output: Contribution to journalArticle

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