Hyaluronic acid-gold nanoparticle/interferon α complex for targeted treatment of hepatitis C virus infection

Min Young Lee, Jeong A. Yang, Ho Sang Jung, Songeun Beack, Jung Eun Choi, Wonhee Hur, Heebeom Koo, Kwang Meyung Kim, Seung Kew Yoon, Sei Kwang Hahn

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Gold nanoparticles (AuNPs) have been extensively investigated as an emerging delivery carrier of various biopharmaceuticals. Instead of nonspecific polyethylene glycol (PEG) conjugated interferon α (IFNα) for the clinical treatment of hepatitis C virus (HCV) infection, in this work, a target-specific long-acting delivery system of IFNα was successfully developed using the hybrid materials of AuNP and hyaluronic acid (HA). The HA-AuNP/IFNα complex was prepared by chemical binding of thiolated HA and physical binding of IFNα to AuNP. According to antiproliferation tests in Daudi cells, the HA-AuNP/IFNα complex showed a comparable biological activity to PEG-Intron with a highly enhanced stability in human serum. Even 7 days postinjection, HA-AuNP/IFNα complex was target-specifically delivered and remained in the murine liver tissue, whereas IFNα and PEG-Intron were not detected in the liver. Accordingly, HA-AuNP/IFNα complex significantly enhanced the expression of 2′,5′-oligoadenylate synthetase 1 (OAS1) for innate immune responses to viral infection in the liver tissue, which was much higher than those by IFNα, PEG-Intron, and AuNP/IFNα complex. Taken together, the target-specific HA-AuNP/IFNα complex was thought to be successfully applied to the systemic treatment of HCV infection.

Original languageEnglish
Pages (from-to)9522-9531
Number of pages10
JournalACS Nano
Volume6
Issue number11
DOIs
Publication statusPublished - 2012 Nov 27
Externally publishedYes

Fingerprint

interferon
hepatitis
Interferons
Hyaluronic acid
viruses
infectious diseases
Hyaluronic Acid
Viruses
Gold
gold
Nanoparticles
nanoparticles
acids
Polyethylene glycols
glycols
polyethylenes
liver
Liver
Introns
delivery

Keywords

  • gold nanoparticle
  • hepatitis C virus
  • hyaluronic acid
  • interferon R
  • targeted delivery

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Lee, M. Y., Yang, J. A., Jung, H. S., Beack, S., Choi, J. E., Hur, W., ... Hahn, S. K. (2012). Hyaluronic acid-gold nanoparticle/interferon α complex for targeted treatment of hepatitis C virus infection. ACS Nano, 6(11), 9522-9531. https://doi.org/10.1021/nn302538y

Hyaluronic acid-gold nanoparticle/interferon α complex for targeted treatment of hepatitis C virus infection. / Lee, Min Young; Yang, Jeong A.; Jung, Ho Sang; Beack, Songeun; Choi, Jung Eun; Hur, Wonhee; Koo, Heebeom; Kim, Kwang Meyung; Yoon, Seung Kew; Hahn, Sei Kwang.

In: ACS Nano, Vol. 6, No. 11, 27.11.2012, p. 9522-9531.

Research output: Contribution to journalArticle

Lee, MY, Yang, JA, Jung, HS, Beack, S, Choi, JE, Hur, W, Koo, H, Kim, KM, Yoon, SK & Hahn, SK 2012, 'Hyaluronic acid-gold nanoparticle/interferon α complex for targeted treatment of hepatitis C virus infection', ACS Nano, vol. 6, no. 11, pp. 9522-9531. https://doi.org/10.1021/nn302538y
Lee, Min Young ; Yang, Jeong A. ; Jung, Ho Sang ; Beack, Songeun ; Choi, Jung Eun ; Hur, Wonhee ; Koo, Heebeom ; Kim, Kwang Meyung ; Yoon, Seung Kew ; Hahn, Sei Kwang. / Hyaluronic acid-gold nanoparticle/interferon α complex for targeted treatment of hepatitis C virus infection. In: ACS Nano. 2012 ; Vol. 6, No. 11. pp. 9522-9531.
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