Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes

Dong Eun Lee, Ah Young Kim, Gurusamy Saravanakumar, Heebeom Koo, Ick Chan Kwon, Kuiwon Choi, Jae Hyung Park, Kwang Meyung Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study developed new enzyme-specific superparamagnetic iron oxide nanoparticles (SPIONs) with potential as a multimodal probe in magnetic resonance (MR) imaging and optical imaging. For the MR/optical dual imaging, SPIONs were coated with near-infrared fluorescence dye (Cy5.5)-labeled hyaluronic acid (Cy5.5-HA), which could be readily degraded by hyaluronidase (HAdase). The presence of HA on the coated SPION was confirmed using FTIR, X-ray diffraction, and thermogravimetric analysis. Physicochemical analyses of Cy5.5-HAcoated SPIONs (Cy5.5-HA-SPIONs) demonstrated their higher colloidal stability and T2 relaxivity than bare SPIONs. When Cy5.5-HA-SPIONs were exposed to the MR instrument for the phantom test, the dark intensity of their T2-weighted image increased in a Fe concentration-dependent manner, suggesting potential as the MR probe. To examine their ability as the enzyme-sensitive optical probe, the fluorescent signals of Cy5.5-HA-SPIONs were measured in the absence or presence of HAdase. In the absence of HAdase, Cy5.5-HA-SPIONs showed a negligible fluorescent signal due to possible quenching between a SPION and fluorescence dye, and self-quenching effect of Cy5.5 molecules. On the other hand, in the presence of HAdase, the fluorescent signal of Cy5.5-HA-SPIONs increased dramatically as a function of the HAdase concentration, resulting from the degradation of the HA backbone. These results suggest that the Cy5.5-HA-SPION is useful as a multimodal nanoprobe for MR imaging and HAdase-sensitive optical imaging.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalMacromolecular Research
Volume19
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1
Externally publishedYes

Fingerprint

Nanoprobes
Hyaluronoglucosaminidase
Magnetic resonance
Iron oxides
Hyaluronic acid
Nanoparticles
Imaging techniques
Hyaluronic Acid
Quenching
ferric oxide
Coloring Agents
Enzymes
Dyes
Fluorescence
CY5.5 cyanine dye
Fluorescent Dyes
X ray diffraction analysis
Thermogravimetric analysis

Keywords

  • Hyaluronic acid
  • Hyaluronidase
  • MR/optical dual imaging
  • SPION

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Chemical Engineering(all)

Cite this

Lee, D. E., Kim, A. Y., Saravanakumar, G., Koo, H., Kwon, I. C., Choi, K., ... Kim, K. M. (2011). Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes. Macromolecular Research, 19(8), 861-867. https://doi.org/10.1007/s13233-011-0804-1

Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes. / Lee, Dong Eun; Kim, Ah Young; Saravanakumar, Gurusamy; Koo, Heebeom; Kwon, Ick Chan; Choi, Kuiwon; Park, Jae Hyung; Kim, Kwang Meyung.

In: Macromolecular Research, Vol. 19, No. 8, 01.08.2011, p. 861-867.

Research output: Contribution to journalArticle

Lee, DE, Kim, AY, Saravanakumar, G, Koo, H, Kwon, IC, Choi, K, Park, JH & Kim, KM 2011, 'Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes', Macromolecular Research, vol. 19, no. 8, pp. 861-867. https://doi.org/10.1007/s13233-011-0804-1
Lee DE, Kim AY, Saravanakumar G, Koo H, Kwon IC, Choi K et al. Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes. Macromolecular Research. 2011 Aug 1;19(8):861-867. https://doi.org/10.1007/s13233-011-0804-1
Lee, Dong Eun ; Kim, Ah Young ; Saravanakumar, Gurusamy ; Koo, Heebeom ; Kwon, Ick Chan ; Choi, Kuiwon ; Park, Jae Hyung ; Kim, Kwang Meyung. / Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes. In: Macromolecular Research. 2011 ; Vol. 19, No. 8. pp. 861-867.
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