Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes

Dong Eun Lee, Ah Young Kim, Gurusamy Saravanakumar, Heebeom Koo, Ick Chan Kwon, Kuiwon Choi, Jae Hyung Park, Kwangmeyung Kim

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20 Citations (Scopus)


This study developed new enzyme-specific superparamagnetic iron oxide nanoparticles (SPIONs) with potential as a multimodal probe in magnetic resonance (MR) imaging and optical imaging. For the MR/optical dual imaging, SPIONs were coated with near-infrared fluorescence dye (Cy5.5)-labeled hyaluronic acid (Cy5.5-HA), which could be readily degraded by hyaluronidase (HAdase). The presence of HA on the coated SPION was confirmed using FTIR, X-ray diffraction, and thermogravimetric analysis. Physicochemical analyses of Cy5.5-HAcoated SPIONs (Cy5.5-HA-SPIONs) demonstrated their higher colloidal stability and T2 relaxivity than bare SPIONs. When Cy5.5-HA-SPIONs were exposed to the MR instrument for the phantom test, the dark intensity of their T2-weighted image increased in a Fe concentration-dependent manner, suggesting potential as the MR probe. To examine their ability as the enzyme-sensitive optical probe, the fluorescent signals of Cy5.5-HA-SPIONs were measured in the absence or presence of HAdase. In the absence of HAdase, Cy5.5-HA-SPIONs showed a negligible fluorescent signal due to possible quenching between a SPION and fluorescence dye, and self-quenching effect of Cy5.5 molecules. On the other hand, in the presence of HAdase, the fluorescent signal of Cy5.5-HA-SPIONs increased dramatically as a function of the HAdase concentration, resulting from the degradation of the HA backbone. These results suggest that the Cy5.5-HA-SPION is useful as a multimodal nanoprobe for MR imaging and HAdase-sensitive optical imaging.

Original languageEnglish
Pages (from-to)861-867
Number of pages7
JournalMacromolecular Research
Issue number8
Publication statusPublished - 2011 Aug


  • Hyaluronic acid
  • Hyaluronidase
  • MR/optical dual imaging

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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    Lee, D. E., Kim, A. Y., Saravanakumar, G., Koo, H., Kwon, I. C., Choi, K., Park, J. H., & Kim, K. (2011). Hyaluronidase-sensitive SPIONs for MR/optical dual imaging nanoprobes. Macromolecular Research, 19(8), 861-867. https://doi.org/10.1007/s13233-011-0804-1