Hydrodynamic effects on bacterial biofilm development in a microfluidic environment

Junghyun Kim, Han Shin Kim, Sewoon Han, Ji Yun Lee, Jae Eung Oh, Seok Chung, Hee-Deung Park

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In aquatic environments, microorganisms tend to form biofilms on surfaces to protect them from harsh conditions. The biofilms then accumulate into multilayered mat-like structures. In this study, we evaluated the effects of the hydrodynamic conditions on the ecology of biofilms produced by Pseudomonas aeruginosa (PA14). In microfluidic channels, we found that the development of biofilms was regulated by hydrodynamic conditions, but the developed biofilms also changed flow velocity by narrowing flow width. The coupled growing conditions were simplified by a new concept of consequent variables, and the dimensionless biofilm development (Ab/h2 & A b/wcs 2) was successfully expressed by the Reynolds number (Re) and the dimension of the channel (r). At low Re, higher flow rates encouraged growth of biofilms, while higher flow rates with high Re suppressed growth of biofilms. These results provide a simple model as a theoretical basis for understanding development of biofilms in microfluidic channels.

Original languageEnglish
Pages (from-to)1846-1849
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume13
Issue number10
DOIs
Publication statusPublished - 2013 May 6

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Microfluidics
Biofilms
Hydrodynamics
Flow rate
Ecology
Growth
Flow velocity
Microorganisms
Pseudomonas aeruginosa
Reynolds number

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Hydrodynamic effects on bacterial biofilm development in a microfluidic environment. / Kim, Junghyun; Kim, Han Shin; Han, Sewoon; Lee, Ji Yun; Oh, Jae Eung; Chung, Seok; Park, Hee-Deung.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 13, No. 10, 06.05.2013, p. 1846-1849.

Research output: Contribution to journalArticle

Kim, Junghyun ; Kim, Han Shin ; Han, Sewoon ; Lee, Ji Yun ; Oh, Jae Eung ; Chung, Seok ; Park, Hee-Deung. / Hydrodynamic effects on bacterial biofilm development in a microfluidic environment. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2013 ; Vol. 13, No. 10. pp. 1846-1849.
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