Hydrogen peroxide-induced VCAM-1 expression in pancreatic islets and β-Cells through extracellular Ca2+ influx

Sukmook Lee, In Su Ha, Jae Hyeon Kim, Kyong Soo Park, Kyu Hyun Han, Sun Hee Kim, Young Chan Chae, Sun Hee Kim, Yun Hee Kim, Pann Ghill Suh, Sung Ho Ryu, Jung Eun Kim, Kitae Bang, Jong-Ik Hwang, Jaeseok Yang, Kwang Wook Park, Junho Chung, Curie Ahn

Research output: Contribution to journalArticle

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Abstract

BACKGROUND.: The use of porcine islets as alternatives to transplantable human islets is hampered by xenotransplant rejection. To identify molecular mechanisms that would allow subversion of xenoislet rejection, we investigated the role of H2O2 in vascular cell adhesion molecule-1 (VCAM-1) expression by porcine and mouse islets and β-cell lines. METHODS.: Porcine islets were treated with H2O2, tumor necrosis factor alpha, interferon-γ, interleukin-1β, and lipopolysaccharide, to assess the effects of inflammatory stimulators on VCAM-1 expression using flow cytometry. The role of Ca in H2O2-induced VCAM-1 expression was investigated in β-cell lines using an extracellular Ca chelator and Ca-depleted media. Furthermore, H2O2-induced VCAM-1 expression was measured in β-cells, pretreated with inhibitors of protein kinase C, phospholipase D, and phosphatidylinositol-3 kinase/Akt. Finally, H2O2-induced VCAM-1 expression was evaluated in porcine islets and rodent β-cell lines infected with an adenovirus encoding catalase, a H2O2-removing enzyme. RESULTS.: H2O2 was most potent inflammatory stimulator of VCAM-1 expression in porcine islets and had the greatest effect on VCAM-1 expression by β-cells. Signaling pathway analysis demonstrated that extracellular Ca influx was critical to H2O2-mediated VCAM-1 expression; however, protein kinase C, phospholipase D, and phosphatidylinositol-3 kinase/Akt activation were not required for VCAM-1 expression. Finally, catalase overexpression inhibited H2O2-induced VCAM-1 expression by islets and β-cell lines. CONCLUSION.: An extracellular calcium-dependent H2O2 pathway is the critical mediator of VCAM-1 expression by pancreatic islets and β-cells. Inhibition of this pathway by catalase overexpression in donor islets can be exploited to protect against xenoislet immune responses.

Original languageEnglish
Pages (from-to)1257-1266
Number of pages10
JournalTransplantation
Volume86
Issue number9
DOIs
Publication statusPublished - 2008 Nov 15

Fingerprint

Vascular Cell Adhesion Molecule-1
Islets of Langerhans
Hydrogen Peroxide
Swine
Phosphatidylinositol 3-Kinase
Catalase
Cell Line
Phospholipase D
Protein Kinase C
Critical Pathways
Chelating Agents
Interleukin-1
Interferon-alpha
Adenoviridae
Lipopolysaccharides
Rodentia
Flow Cytometry
Tumor Necrosis Factor-alpha
Calcium

Keywords

  • Calcium
  • Catalase
  • Hydrogen peroxide
  • Pancreaticβ-cells
  • VCAM-1

ASJC Scopus subject areas

  • Transplantation

Cite this

Hydrogen peroxide-induced VCAM-1 expression in pancreatic islets and β-Cells through extracellular Ca2+ influx. / Lee, Sukmook; Ha, In Su; Kim, Jae Hyeon; Park, Kyong Soo; Han, Kyu Hyun; Kim, Sun Hee; Chae, Young Chan; Kim, Sun Hee; Kim, Yun Hee; Suh, Pann Ghill; Ryu, Sung Ho; Kim, Jung Eun; Bang, Kitae; Hwang, Jong-Ik; Yang, Jaeseok; Park, Kwang Wook; Chung, Junho; Ahn, Curie.

In: Transplantation, Vol. 86, No. 9, 15.11.2008, p. 1257-1266.

Research output: Contribution to journalArticle

Lee, S, Ha, IS, Kim, JH, Park, KS, Han, KH, Kim, SH, Chae, YC, Kim, SH, Kim, YH, Suh, PG, Ryu, SH, Kim, JE, Bang, K, Hwang, J-I, Yang, J, Park, KW, Chung, J & Ahn, C 2008, 'Hydrogen peroxide-induced VCAM-1 expression in pancreatic islets and β-Cells through extracellular Ca2+ influx', Transplantation, vol. 86, no. 9, pp. 1257-1266. https://doi.org/10.1097/TP.0b013e318188ab04
Lee, Sukmook ; Ha, In Su ; Kim, Jae Hyeon ; Park, Kyong Soo ; Han, Kyu Hyun ; Kim, Sun Hee ; Chae, Young Chan ; Kim, Sun Hee ; Kim, Yun Hee ; Suh, Pann Ghill ; Ryu, Sung Ho ; Kim, Jung Eun ; Bang, Kitae ; Hwang, Jong-Ik ; Yang, Jaeseok ; Park, Kwang Wook ; Chung, Junho ; Ahn, Curie. / Hydrogen peroxide-induced VCAM-1 expression in pancreatic islets and β-Cells through extracellular Ca2+ influx. In: Transplantation. 2008 ; Vol. 86, No. 9. pp. 1257-1266.
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AU - Lee, Sukmook

AU - Ha, In Su

AU - Kim, Jae Hyeon

AU - Park, Kyong Soo

AU - Han, Kyu Hyun

AU - Kim, Sun Hee

AU - Chae, Young Chan

AU - Kim, Sun Hee

AU - Kim, Yun Hee

AU - Suh, Pann Ghill

AU - Ryu, Sung Ho

AU - Kim, Jung Eun

AU - Bang, Kitae

AU - Hwang, Jong-Ik

AU - Yang, Jaeseok

AU - Park, Kwang Wook

AU - Chung, Junho

AU - Ahn, Curie

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N2 - BACKGROUND.: The use of porcine islets as alternatives to transplantable human islets is hampered by xenotransplant rejection. To identify molecular mechanisms that would allow subversion of xenoislet rejection, we investigated the role of H2O2 in vascular cell adhesion molecule-1 (VCAM-1) expression by porcine and mouse islets and β-cell lines. METHODS.: Porcine islets were treated with H2O2, tumor necrosis factor alpha, interferon-γ, interleukin-1β, and lipopolysaccharide, to assess the effects of inflammatory stimulators on VCAM-1 expression using flow cytometry. The role of Ca in H2O2-induced VCAM-1 expression was investigated in β-cell lines using an extracellular Ca chelator and Ca-depleted media. Furthermore, H2O2-induced VCAM-1 expression was measured in β-cells, pretreated with inhibitors of protein kinase C, phospholipase D, and phosphatidylinositol-3 kinase/Akt. Finally, H2O2-induced VCAM-1 expression was evaluated in porcine islets and rodent β-cell lines infected with an adenovirus encoding catalase, a H2O2-removing enzyme. RESULTS.: H2O2 was most potent inflammatory stimulator of VCAM-1 expression in porcine islets and had the greatest effect on VCAM-1 expression by β-cells. Signaling pathway analysis demonstrated that extracellular Ca influx was critical to H2O2-mediated VCAM-1 expression; however, protein kinase C, phospholipase D, and phosphatidylinositol-3 kinase/Akt activation were not required for VCAM-1 expression. Finally, catalase overexpression inhibited H2O2-induced VCAM-1 expression by islets and β-cell lines. CONCLUSION.: An extracellular calcium-dependent H2O2 pathway is the critical mediator of VCAM-1 expression by pancreatic islets and β-cells. Inhibition of this pathway by catalase overexpression in donor islets can be exploited to protect against xenoislet immune responses.

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KW - Catalase

KW - Hydrogen peroxide

KW - Pancreaticβ-cells

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