Hydrogen peroxide induces the death astrocytes through the down-regulation of the constitutive nuclear factor-kappaB activity

Jung Jin Choi, JiWoong Choi, Chi Dug Kang, Xia Chen, Chun Fu Wu, Kwang Ho Ko, Won-Ki Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nuclear factor-kappaB (NF-κB) has a dual role in the promotion or attenuation of cell death. Here, we demonstrated the role of NF-κB in the H2O2-induced death of astrocytes. H2O2 evoked the release of lactate dehydrogenase (LDH), a marker of cell death, and concomitantly decreased the DNA binding and transcriptional activity of NF-κ3ba;B in cultured astrocytes. H2O2-induced astrocyte death was markedly increased by the co-treatment with pyrrolidinedithiocarbamate, an NF-κB inhibitor. Moreover, the elevation of constitutive NF-κB activity by overexpressing p65 NF-κB subunit attenuated H2O2 toxicity, whereas NF-κB inhibition by overexpressing Iκba;B potentiated the toxicity. NF-κB activity and H2O2 cytotoxicity was further found to be dependent on cell density. Compared with astrocytes in low cell density, those in high cell density exhibited a higher constitutive NF-κB activity and a stronger resistance to H2O2 cytotoxicity. These results indicate that the constitutive activity of NF-κB in astrocytes is required for their survival under oxidative stress such as H2O2.

Original languageEnglish
Pages (from-to)555-562
Number of pages8
JournalFree Radical Research
Volume41
Issue number5
DOIs
Publication statusPublished - 2007 Apr 8
Externally publishedYes

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Keywords

  • Astrocyte
  • HO
  • LDH release
  • NF-κBB

ASJC Scopus subject areas

  • Biochemistry

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