Hydrogen production from biodiesel byproduct by immobilized Enterobacter aerogenes

Jinmi Han, Dohoon Lee, Jinku Cho, Jeewon Lee, Sangyong Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The recent rapid growth of the biodiesel industry has generated a significant amount of glycerol as a byproduct. As a result, the price of glycerol is currently relatively low, making it an attractive starting material for the production of chemicals with higher values. Crude glycerol can be directly converted through microbial fermentation into various chemicals such as hydrogen. In this study, we optimized immobilization of a facultative hydrogen producing microorganism, Enterobacter aerogenes, with the goal of developing biocatalysts that was appropriate for the continuous hydrogen production from glycerol. Several carriers were tested and agar was found to be the most effective. In addition, it was clearly shown that variables such as the carrier content and cell loading should be controlled for the immobilization of biocatalysts with high hydrogen productivity, stability, and reusability. After optimization of these variables, we were able to obtain reusable biocatalysts that could directly convert the byproduct stream from biodiesel processes into hydrogen in continuous processes.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume35
Issue number1-2
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Enterobacter aerogenes
Biofuels
Hydrogen production
Biodiesel
Glycerol
Biocatalysts
Byproducts
Hydrogen
Enzymes
Immobilization
Reusability
Microorganisms
Fermentation
Agar
Productivity
Industry
Growth

Keywords

  • Agar
  • Biodiesel
  • Enterobacter aerogenes
  • Hydrogen
  • Immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Hydrogen production from biodiesel byproduct by immobilized Enterobacter aerogenes. / Han, Jinmi; Lee, Dohoon; Cho, Jinku; Lee, Jeewon; Kim, Sangyong.

In: Bioprocess and Biosystems Engineering, Vol. 35, No. 1-2, 01.01.2012, p. 151-157.

Research output: Contribution to journalArticle

Han, Jinmi ; Lee, Dohoon ; Cho, Jinku ; Lee, Jeewon ; Kim, Sangyong. / Hydrogen production from biodiesel byproduct by immobilized Enterobacter aerogenes. In: Bioprocess and Biosystems Engineering. 2012 ; Vol. 35, No. 1-2. pp. 151-157.
@article{5bd0d9437c564fd6b53e2a5e369126a6,
title = "Hydrogen production from biodiesel byproduct by immobilized Enterobacter aerogenes",
abstract = "The recent rapid growth of the biodiesel industry has generated a significant amount of glycerol as a byproduct. As a result, the price of glycerol is currently relatively low, making it an attractive starting material for the production of chemicals with higher values. Crude glycerol can be directly converted through microbial fermentation into various chemicals such as hydrogen. In this study, we optimized immobilization of a facultative hydrogen producing microorganism, Enterobacter aerogenes, with the goal of developing biocatalysts that was appropriate for the continuous hydrogen production from glycerol. Several carriers were tested and agar was found to be the most effective. In addition, it was clearly shown that variables such as the carrier content and cell loading should be controlled for the immobilization of biocatalysts with high hydrogen productivity, stability, and reusability. After optimization of these variables, we were able to obtain reusable biocatalysts that could directly convert the byproduct stream from biodiesel processes into hydrogen in continuous processes.",
keywords = "Agar, Biodiesel, Enterobacter aerogenes, Hydrogen, Immobilization",
author = "Jinmi Han and Dohoon Lee and Jinku Cho and Jeewon Lee and Sangyong Kim",
year = "2012",
month = "1",
day = "1",
doi = "10.1007/s00449-011-0593-0",
language = "English",
volume = "35",
pages = "151--157",
journal = "Bioprocess and Biosystems Engineering",
issn = "1615-7591",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - Hydrogen production from biodiesel byproduct by immobilized Enterobacter aerogenes

AU - Han, Jinmi

AU - Lee, Dohoon

AU - Cho, Jinku

AU - Lee, Jeewon

AU - Kim, Sangyong

PY - 2012/1/1

Y1 - 2012/1/1

N2 - The recent rapid growth of the biodiesel industry has generated a significant amount of glycerol as a byproduct. As a result, the price of glycerol is currently relatively low, making it an attractive starting material for the production of chemicals with higher values. Crude glycerol can be directly converted through microbial fermentation into various chemicals such as hydrogen. In this study, we optimized immobilization of a facultative hydrogen producing microorganism, Enterobacter aerogenes, with the goal of developing biocatalysts that was appropriate for the continuous hydrogen production from glycerol. Several carriers were tested and agar was found to be the most effective. In addition, it was clearly shown that variables such as the carrier content and cell loading should be controlled for the immobilization of biocatalysts with high hydrogen productivity, stability, and reusability. After optimization of these variables, we were able to obtain reusable biocatalysts that could directly convert the byproduct stream from biodiesel processes into hydrogen in continuous processes.

AB - The recent rapid growth of the biodiesel industry has generated a significant amount of glycerol as a byproduct. As a result, the price of glycerol is currently relatively low, making it an attractive starting material for the production of chemicals with higher values. Crude glycerol can be directly converted through microbial fermentation into various chemicals such as hydrogen. In this study, we optimized immobilization of a facultative hydrogen producing microorganism, Enterobacter aerogenes, with the goal of developing biocatalysts that was appropriate for the continuous hydrogen production from glycerol. Several carriers were tested and agar was found to be the most effective. In addition, it was clearly shown that variables such as the carrier content and cell loading should be controlled for the immobilization of biocatalysts with high hydrogen productivity, stability, and reusability. After optimization of these variables, we were able to obtain reusable biocatalysts that could directly convert the byproduct stream from biodiesel processes into hydrogen in continuous processes.

KW - Agar

KW - Biodiesel

KW - Enterobacter aerogenes

KW - Hydrogen

KW - Immobilization

UR - http://www.scopus.com/inward/record.url?scp=84857452787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857452787&partnerID=8YFLogxK

U2 - 10.1007/s00449-011-0593-0

DO - 10.1007/s00449-011-0593-0

M3 - Article

C2 - 21915673

AN - SCOPUS:84857452787

VL - 35

SP - 151

EP - 157

JO - Bioprocess and Biosystems Engineering

JF - Bioprocess and Biosystems Engineering

SN - 1615-7591

IS - 1-2

ER -