Hydrogen production of the hyperthermophilic eubacterium, Thermotoga neapolitana under N2 sparging condition

Tam Anh D Nguyen, Se Jong Han, Jun Pyo Kim, Mi Sun Kim, Sang Jun Sim

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Gas sparging was found to be a useful technique to reduce hydrogen partial pressure in the liquid phase to enhance the hydrogen yields of strictly anaerobically fermentative bacteria. The effect of nitrogen (N2) sparging on hydrogen yield was investigated in sterile and non-sterile conditions using a pure strain of the hyperthermophilic eubacteria, Thermotoga neapolitana with glucose or xylose as a carbon source. The maximum hydrogen accumulations reached 41% of the gaseous mixtures after 30-40 h. Two applications of N2 sparging after the H2 content in the headspace reached the maximum levels gave an increase of H2 production by 78% from 1.82 to 3.24 mol H2/mol glucose and by 56% from 1.41 to 2.20 mol H2/mol xylose. This result suggested that the removal of the produced H2 from the gas headspace of the limited-volume, closed culture vial when it achieves the maximum level of H2 tolerance of the bacterium is a necessary technique to improve its H2 yield.

Original languageEnglish
JournalBioresource Technology
Volume101
Issue number1 SUPPL.
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Fingerprint

Hydrogen production
Hydrogen
hydrogen
Xylose
Glucose
Bacteria
glucose
Gases
bacterium
partial pressure
gas
Partial pressure
Nitrogen
Carbon
tolerance
liquid
nitrogen
carbon
Liquids

Keywords

  • Gas sparging
  • Hydrogen production
  • Hyperthermophilic eubacteria
  • Thermotoga neapolitana

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Hydrogen production of the hyperthermophilic eubacterium, Thermotoga neapolitana under N2 sparging condition. / Nguyen, Tam Anh D; Han, Se Jong; Kim, Jun Pyo; Kim, Mi Sun; Sim, Sang Jun.

In: Bioresource Technology, Vol. 101, No. 1 SUPPL., 01.01.2010.

Research output: Contribution to journalArticle

Nguyen, Tam Anh D ; Han, Se Jong ; Kim, Jun Pyo ; Kim, Mi Sun ; Sim, Sang Jun. / Hydrogen production of the hyperthermophilic eubacterium, Thermotoga neapolitana under N2 sparging condition. In: Bioresource Technology. 2010 ; Vol. 101, No. 1 SUPPL.
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