Abstract
Hydrogen sulfide (H2S) functions as a physiological gas transmitter in both normal and pathophysiological cellular events. H2S is produced from substances by three enzymes: cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST). In human tissues, these enzymes are involved in tissue-specific biochemical pathways for H2S production. For example, CBS and cysteine aminotransferase/MST are present in the brain, but CSE is not. Thus, we examined the expression of H2S production-related enzymes in peripheral nerves. Here, we found that CSE and MST/cysteine aminotransferase, but not CBS, were present in normal peripheral nerves. In addition, injured sciatic nerves in vivo up-regulated CSE in Schwann cells during Wallerian degeneration (WD); however, CSE was not up-regulated in peripheral axons. Using an ex vivo sciatic nerve explant culture, we found that the inhibition of H2S production broadly prevented the process of nerve degeneration, including myelin fragmentation, axonal degradation, Schwann cell dedifferentiation, and Schwann cell proliferation in vitro and in vivo. Thus, these results indicate that H2S signaling is essential for Schwann cell responses to peripheral nerve injury.
| Original language | English |
|---|---|
| Pages (from-to) | 230-242 |
| Number of pages | 13 |
| Journal | Journal of Neurochemistry |
| Volume | 132 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2015 Jan 1 |
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Keywords
- axonal degradation
- cytstathionine-γ-lyase (CSE)
- demyelination
- hydrogen sulfide
- Schwann cells
- Wallerian degeneration
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
Hydrogen sulfide is essential for Schwann cell responses to peripheral nerve injury. / Park, Byung Sun; Kim, Hyun Wook; Rhyu, Im Joo; Park, Chan; Yeo, Seung Geun; Huh, Youngbuhm; Jeong, Na Young; Jung, Junyang.
In: Journal of Neurochemistry, Vol. 132, No. 2, 01.01.2015, p. 230-242.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hydrogen sulfide is essential for Schwann cell responses to peripheral nerve injury
AU - Park, Byung Sun
AU - Kim, Hyun Wook
AU - Rhyu, Im Joo
AU - Park, Chan
AU - Yeo, Seung Geun
AU - Huh, Youngbuhm
AU - Jeong, Na Young
AU - Jung, Junyang
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Hydrogen sulfide (H2S) functions as a physiological gas transmitter in both normal and pathophysiological cellular events. H2S is produced from substances by three enzymes: cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST). In human tissues, these enzymes are involved in tissue-specific biochemical pathways for H2S production. For example, CBS and cysteine aminotransferase/MST are present in the brain, but CSE is not. Thus, we examined the expression of H2S production-related enzymes in peripheral nerves. Here, we found that CSE and MST/cysteine aminotransferase, but not CBS, were present in normal peripheral nerves. In addition, injured sciatic nerves in vivo up-regulated CSE in Schwann cells during Wallerian degeneration (WD); however, CSE was not up-regulated in peripheral axons. Using an ex vivo sciatic nerve explant culture, we found that the inhibition of H2S production broadly prevented the process of nerve degeneration, including myelin fragmentation, axonal degradation, Schwann cell dedifferentiation, and Schwann cell proliferation in vitro and in vivo. Thus, these results indicate that H2S signaling is essential for Schwann cell responses to peripheral nerve injury.
AB - Hydrogen sulfide (H2S) functions as a physiological gas transmitter in both normal and pathophysiological cellular events. H2S is produced from substances by three enzymes: cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (MST). In human tissues, these enzymes are involved in tissue-specific biochemical pathways for H2S production. For example, CBS and cysteine aminotransferase/MST are present in the brain, but CSE is not. Thus, we examined the expression of H2S production-related enzymes in peripheral nerves. Here, we found that CSE and MST/cysteine aminotransferase, but not CBS, were present in normal peripheral nerves. In addition, injured sciatic nerves in vivo up-regulated CSE in Schwann cells during Wallerian degeneration (WD); however, CSE was not up-regulated in peripheral axons. Using an ex vivo sciatic nerve explant culture, we found that the inhibition of H2S production broadly prevented the process of nerve degeneration, including myelin fragmentation, axonal degradation, Schwann cell dedifferentiation, and Schwann cell proliferation in vitro and in vivo. Thus, these results indicate that H2S signaling is essential for Schwann cell responses to peripheral nerve injury.
KW - axonal degradation
KW - cytstathionine-γ-lyase (CSE)
KW - demyelination
KW - hydrogen sulfide
KW - Schwann cells
KW - Wallerian degeneration
UR - http://www.scopus.com/inward/record.url?scp=84921374077&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84921374077&partnerID=8YFLogxK
U2 - 10.1111/jnc.12932
DO - 10.1111/jnc.12932
M3 - Article
C2 - 25123509
AN - SCOPUS:84921374077
VL - 132
SP - 230
EP - 242
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 2
ER -