Hydrogenophaga crassostreae sp. nov., isolated from a Pacific oyster

Chaeyun Baek, Eunji Kim, Su Kyoung Shin, Sungmi Choi, Hana Yi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A Gram-negative, motile, rod-shaped, and aerobic bacterial strain, designated LPB0072T, was isolated from a Pacific oyster (Crassostrea gigas). Autotrophic growth with hydrogen gas was not observed. Cells oxidized thiosulfate to sulfate and reduced nitrate to nitrite. The complete genome sequence of strain LPB0072T (CP017476) is 4.94 Mb in length and contains 4459 protein-coding genes, with a G+C content of 61.3 mol%. Analysis of the 16S rRNA gene sequence indicated that strain LPB0072T belongs to the genus Hydrogenophaga, with greatest sequence similarity to the type strain of Hydrogenophaga taeniospiralis (97.5 %). The isoprenoid quinone (Q-8) and the major cellular fatty acids (C16 : 1ω7c and/or C16 : 1ω6c, C16 : 0 and C17 : 1ω6c) identified were concordant with the chemotaxonomic properties of the genus Hydrogenophaga. The average nucleotide identities with closely related species were below the suggested boundary for species delineation, indicating that the isolate is a novel species. Numerous physiological and biochemical features also distinguished the isolate from other known Hydrogenophaga species. Based on the polyphasic data presented in this study, strain LPB0072T should be classified as a novel species in the genus Hydrogenophaga, and the name Hydrogenophaga crassostreae sp. nov. is proposed. The type strain is LPB0072T (=KACC 18705T=JCM 31188T).

Original languageEnglish
Article number002244
Pages (from-to)4045-4049
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number10
DOIs
Publication statusPublished - 2017 Oct 1

Fingerprint

Hydrogenophaga
Comamonadaceae
Crassostrea
Ostreidae
Crassostrea gigas
new species
Hydrogenophaga taeniospiralis
Autotrophic Processes
gene
thiosulfate
isoprenoid
Thiosulfates
thiosulfates
isoprenoids
Terpenes
nitrite
Nitrites
quinones
rRNA Genes
fatty acid

Keywords

  • Novel species
  • Pacific oyster
  • Sequence analysis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Hydrogenophaga crassostreae sp. nov., isolated from a Pacific oyster. / Baek, Chaeyun; Kim, Eunji; Shin, Su Kyoung; Choi, Sungmi; Yi, Hana.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 10, 002244, 01.10.2017, p. 4045-4049.

Research output: Contribution to journalArticle

Baek, Chaeyun ; Kim, Eunji ; Shin, Su Kyoung ; Choi, Sungmi ; Yi, Hana. / Hydrogenophaga crassostreae sp. nov., isolated from a Pacific oyster. In: International Journal of Systematic and Evolutionary Microbiology. 2017 ; Vol. 67, No. 10. pp. 4045-4049.
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