Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans

Sang D uck Jeon, Su J ung Kim, Sung H yun Park, Gi Wook Choi, Sung Ok Han

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The role of the scaffolding proteins, cellulose binding protein B and C (CbpB and CbpC, respectively) were identified in cellulolytic complex (cellulosome) of Clostridium cellulovorans for efficient degradation of cellulose. Recombinant CbpB and CbpC directly anchored to the cell surface of C. cellulovorans. In addition, CbpB and CbpC showed increased hydrolytic activity on crystalline cellulose incubated with exoglucanase S (ExgS) and endoglucanase Z (EngZ) compared with the activity of free enzymes. Moreover, the results showed synergistic effects of crystalline cellulose hydrolytic activity (1.8- to 2.2-fold) when CbpB and CbpC complex with ExgS and EngZ are incubated with cellulolytic complex containing mini-CbpA. The results suggest C. cellulovorans critically uses CbpB and CbpC, which can directly anchor cells for the hydrolysis of cellulosic material with the major cellulosome complex.

Original languageEnglish
Pages (from-to)505-511
Number of pages7
JournalBioresource Technology
Volume191
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Clostridium cellulovorans
Clostridium
Cellulose
cellulose
Cellulosomes
Crystalline materials
Proteins
protein
Protein C
Anchors
anchor
Protein Binding
hydrolysis
Hydrolysis
Carrier Proteins
Enzymes
enzyme
fold
Degradation
degradation

Keywords

  • Cellulose hydrolysis
  • Cellulosome
  • Clostridium cellulovorans
  • Scaffolding protein
  • Synergistic effect

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hydrolytic effects of scaffolding proteins CbpB and CbpC on crystalline cellulose mediated by the major cellulolytic complex from Clostridium cellulovorans. / Jeon, Sang D uck; Kim, Su J ung; Park, Sung H yun; Choi, Gi Wook; Han, Sung Ok.

In: Bioresource Technology, Vol. 191, 01.09.2015, p. 505-511.

Research output: Contribution to journalArticle

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