Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip

Ho Lee Kwang, Han Kwon Gu, Jung Shin Su, Ju Yeoul Baek, Keun Han Dong, Yongdoo Park, Sang Hoon Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mimicking cellular microenvironments by MEMS technology is one of the emerging research areas. Integrated biomimetic systems with nanofiber polymer networks and microfluidic chips were fabricated and cellular behaviors were observed by changing surface characteristics of nano-fibers and flow rates of microchannels. Modification of polyurethane nanofiber surfaces were achieved by grafting acrylic acid with plasma treatment and these nanofiber matrices were employed in a poly(dimethylsiloxane) based microfluidic chip. The surface characteristics of both electrospun nanofiber matrices was evaluated by measuring contact angle, porosity, and chemical structure using attenuated total reflection-Fourier transform infrared spectrometry. After modification, a terminal carboxyl group formed on the nanofiber surface and the wettability increased significantly. Human MSCs were seeded on the nanofiber matrices and a morphological investigation with actin filament staining and scanning electron microscopy was performed. A proliferation test by WST-1 and Live/Dead assay were performed to investigate the cell culture environment. It was observed that the cells on the AA-grafted nanofibers spread and proliferate compared to untreated nanofibers. It has also shown that flow rates in the microchannels played an important role for cell proliferation (Sim et al., Lab Chip 2007;7:1775-1782). Integration of nanofiber matrices into the microchannels provides the useful tools for mimicking cellular microenvironments and elucidating basic questions of cell and ECM assembly and interactions.

Original languageEnglish
Pages (from-to)619-628
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume90
Issue number2
DOIs
Publication statusPublished - 2009 Aug 1

Fingerprint

Polyurethanes
Nanofibers
Microfluidics
Microchannels
Flow rate
Military electronic countermeasures
Cell proliferation
Biomimetics
Polydimethylsiloxane
Cell culture
Spectrometry
Acrylics
Contact angle
MEMS
Wetting
Actins
Assays
Fourier transforms
Polymers
Porosity

Keywords

  • Cell chip
  • Electrospun nanofiber
  • Human mesenchymal stem cells
  • Polydimethylsiloxane
  • Polyurethane

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip. / Kwang, Ho Lee; Gu, Han Kwon; Su, Jung Shin; Baek, Ju Yeoul; Dong, Keun Han; Park, Yongdoo; Lee, Sang Hoon.

In: Journal of Biomedical Materials Research - Part A, Vol. 90, No. 2, 01.08.2009, p. 619-628.

Research output: Contribution to journalArticle

Kwang, Ho Lee ; Gu, Han Kwon ; Su, Jung Shin ; Baek, Ju Yeoul ; Dong, Keun Han ; Park, Yongdoo ; Lee, Sang Hoon. / Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip. In: Journal of Biomedical Materials Research - Part A. 2009 ; Vol. 90, No. 2. pp. 619-628.
@article{839bc99b348d4a7a9285550d4f2eb39b,
title = "Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip",
abstract = "Mimicking cellular microenvironments by MEMS technology is one of the emerging research areas. Integrated biomimetic systems with nanofiber polymer networks and microfluidic chips were fabricated and cellular behaviors were observed by changing surface characteristics of nano-fibers and flow rates of microchannels. Modification of polyurethane nanofiber surfaces were achieved by grafting acrylic acid with plasma treatment and these nanofiber matrices were employed in a poly(dimethylsiloxane) based microfluidic chip. The surface characteristics of both electrospun nanofiber matrices was evaluated by measuring contact angle, porosity, and chemical structure using attenuated total reflection-Fourier transform infrared spectrometry. After modification, a terminal carboxyl group formed on the nanofiber surface and the wettability increased significantly. Human MSCs were seeded on the nanofiber matrices and a morphological investigation with actin filament staining and scanning electron microscopy was performed. A proliferation test by WST-1 and Live/Dead assay were performed to investigate the cell culture environment. It was observed that the cells on the AA-grafted nanofibers spread and proliferate compared to untreated nanofibers. It has also shown that flow rates in the microchannels played an important role for cell proliferation (Sim et al., Lab Chip 2007;7:1775-1782). Integration of nanofiber matrices into the microchannels provides the useful tools for mimicking cellular microenvironments and elucidating basic questions of cell and ECM assembly and interactions.",
keywords = "Cell chip, Electrospun nanofiber, Human mesenchymal stem cells, Polydimethylsiloxane, Polyurethane",
author = "Kwang, {Ho Lee} and Gu, {Han Kwon} and Su, {Jung Shin} and Baek, {Ju Yeoul} and Dong, {Keun Han} and Yongdoo Park and Lee, {Sang Hoon}",
year = "2009",
month = "8",
day = "1",
doi = "10.1002/jbm.a.32059",
language = "English",
volume = "90",
pages = "619--628",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "2",

}

TY - JOUR

T1 - Hydrophilic electrospun polyurethane nanofiber matrices for hMSC culture in a microfluidic cell chip

AU - Kwang, Ho Lee

AU - Gu, Han Kwon

AU - Su, Jung Shin

AU - Baek, Ju Yeoul

AU - Dong, Keun Han

AU - Park, Yongdoo

AU - Lee, Sang Hoon

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Mimicking cellular microenvironments by MEMS technology is one of the emerging research areas. Integrated biomimetic systems with nanofiber polymer networks and microfluidic chips were fabricated and cellular behaviors were observed by changing surface characteristics of nano-fibers and flow rates of microchannels. Modification of polyurethane nanofiber surfaces were achieved by grafting acrylic acid with plasma treatment and these nanofiber matrices were employed in a poly(dimethylsiloxane) based microfluidic chip. The surface characteristics of both electrospun nanofiber matrices was evaluated by measuring contact angle, porosity, and chemical structure using attenuated total reflection-Fourier transform infrared spectrometry. After modification, a terminal carboxyl group formed on the nanofiber surface and the wettability increased significantly. Human MSCs were seeded on the nanofiber matrices and a morphological investigation with actin filament staining and scanning electron microscopy was performed. A proliferation test by WST-1 and Live/Dead assay were performed to investigate the cell culture environment. It was observed that the cells on the AA-grafted nanofibers spread and proliferate compared to untreated nanofibers. It has also shown that flow rates in the microchannels played an important role for cell proliferation (Sim et al., Lab Chip 2007;7:1775-1782). Integration of nanofiber matrices into the microchannels provides the useful tools for mimicking cellular microenvironments and elucidating basic questions of cell and ECM assembly and interactions.

AB - Mimicking cellular microenvironments by MEMS technology is one of the emerging research areas. Integrated biomimetic systems with nanofiber polymer networks and microfluidic chips were fabricated and cellular behaviors were observed by changing surface characteristics of nano-fibers and flow rates of microchannels. Modification of polyurethane nanofiber surfaces were achieved by grafting acrylic acid with plasma treatment and these nanofiber matrices were employed in a poly(dimethylsiloxane) based microfluidic chip. The surface characteristics of both electrospun nanofiber matrices was evaluated by measuring contact angle, porosity, and chemical structure using attenuated total reflection-Fourier transform infrared spectrometry. After modification, a terminal carboxyl group formed on the nanofiber surface and the wettability increased significantly. Human MSCs were seeded on the nanofiber matrices and a morphological investigation with actin filament staining and scanning electron microscopy was performed. A proliferation test by WST-1 and Live/Dead assay were performed to investigate the cell culture environment. It was observed that the cells on the AA-grafted nanofibers spread and proliferate compared to untreated nanofibers. It has also shown that flow rates in the microchannels played an important role for cell proliferation (Sim et al., Lab Chip 2007;7:1775-1782). Integration of nanofiber matrices into the microchannels provides the useful tools for mimicking cellular microenvironments and elucidating basic questions of cell and ECM assembly and interactions.

KW - Cell chip

KW - Electrospun nanofiber

KW - Human mesenchymal stem cells

KW - Polydimethylsiloxane

KW - Polyurethane

UR - http://www.scopus.com/inward/record.url?scp=67650485298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650485298&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.32059

DO - 10.1002/jbm.a.32059

M3 - Article

VL - 90

SP - 619

EP - 628

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 2

ER -