Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel

Jong Ho Kim, Yoo Shin Kim, Sungwon Kim, Jae Hyung Park, Kwang Meyung Kim, Kuiwon Choi, Hesson Chung, Seo Young Jeong, Rang Woon Park, In-San Kim, Ick Chan Kwon

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

Self-assembled nanoparticles based on hydrophobically modified glycol chitosan (HGC) were prepared as a carrier for paclitaxel. HGC conjugates were prepared by chemically linking 5β-cholanic acid to glycol chitosan chains using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide chemistry. In phosphate-buffered saline (PBS; pH 7.4), the synthesized HGC conjugates formed nano-sized particles with a diameter of 200 nm and exhibited high thermodynamic stability as reflected by their low critical aggregation concentration (0.03 mg/ml). Paclitaxel was efficiently loaded into HGC nanoparticles up to 10 wt.% using a dialysis method. The paclitaxel-loaded HGC (PTX-HGC) nanoparticles were 400 nm in diameter and were stable in PBS for 10 days. These PTX-HGC nanoparticles also showed sustained release of the incorporated of paclitaxel (80% of the loaded dose was released in 8 days at 37°C in PBS). Owing to sustained release, the PTX-HGC nanoparticles were less cytotoxic to B16F10 melanoma cells than free paclitaxel formulated in Cremophor EL. Injection of PTX-HGC nanoparticles into the tail vein of tumor-bearing mice prevented increases in tumor volume for 8 days. Finally, PTX was less toxic to the tumor-bearing mice when formulated in HGC nanoparticles than when formulated with Cremophor EL.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalJournal of Controlled Release
Volume111
Issue number1-2
DOIs
Publication statusPublished - 2006 Mar 10
Externally publishedYes

Fingerprint

Paclitaxel
Nanoparticles
Ethyldimethylaminopropyl Carbodiimide
glycol-chitosan
Poisons
Tumor Burden
Thermodynamics
Tail
Dialysis
Veins
Melanoma
Neoplasms
Phosphates
Injections

Keywords

  • Hydrophobically modified glycol chitosan
  • In vivo anti-tumor effect
  • Nanoparticles
  • Paclitaxel

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel. / Kim, Jong Ho; Kim, Yoo Shin; Kim, Sungwon; Park, Jae Hyung; Kim, Kwang Meyung; Choi, Kuiwon; Chung, Hesson; Jeong, Seo Young; Park, Rang Woon; Kim, In-San; Kwon, Ick Chan.

In: Journal of Controlled Release, Vol. 111, No. 1-2, 10.03.2006, p. 228-234.

Research output: Contribution to journalArticle

Kim, JH, Kim, YS, Kim, S, Park, JH, Kim, KM, Choi, K, Chung, H, Jeong, SY, Park, RW, Kim, I-S & Kwon, IC 2006, 'Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel', Journal of Controlled Release, vol. 111, no. 1-2, pp. 228-234. https://doi.org/10.1016/j.jconrel.2005.12.013
Kim, Jong Ho ; Kim, Yoo Shin ; Kim, Sungwon ; Park, Jae Hyung ; Kim, Kwang Meyung ; Choi, Kuiwon ; Chung, Hesson ; Jeong, Seo Young ; Park, Rang Woon ; Kim, In-San ; Kwon, Ick Chan. / Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel. In: Journal of Controlled Release. 2006 ; Vol. 111, No. 1-2. pp. 228-234.
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