Hydrophobically modified polysaccharide-based on polysialic acid nanoparticles as carriers for anticancer drugs

Bom Jung, Man Kyu Shim, Min Ju Park, Eun Hyang Jang, Hong Yeol Yoon, Kwang Meyung Kim, Jong Ho Kim

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study presented the development of hydrophobically modified polysialic acid (HPSA) nanoparticles, a novel anticancer drug nanocarrier that increases therapeutic efficacy without causing nonspecific toxicity towards normal cells. HPSA nanoparticles were prepared by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling between N-deacetylated polysialic acid (PSA) and 5β-cholanic acid. The physicochemical characteristics of HPSA nanoparticles (zeta-potential, morphology and size) were measured, and in vitro cytotoxicity and cellular uptake of PSA and HPSA nanoparticles were tested in A549 cells. In vivo cancer targeting of HPSA nanoparticles was evaluated by labeling PSA and HPSA nanoparticles with Cy5.5, a near-infrared fluorescent dye, for imaging. HPSA nanoparticles showed improved cancer-targeting ability compared with PSA. Doxorubicin-loaded HPSA (DOX-HPSA) nanoparticles were prepared using a simple dialysis method. An analysis of the in vitro drug-release profile and drug-delivery behavior showed that DOX was effectively released from DOX-HPSA nanoparticles. In vivo cancer therapy with DOX-HPSA nanoparticles in mice showed antitumor effects that resembled those of free DOX. Moreover, DOX-HPSA nanoparticles had low toxicity toward other organs, reflecting their tumor-targeting property. Hence, HPSA nanoparticles are considered a potential nanocarrier for anticancer agents.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalInternational Journal of Pharmaceutics
Volume520
Issue number1-2
DOIs
Publication statusPublished - 2017 Mar 30
Externally publishedYes

Keywords

  • Doxorubicin
  • EPR effect
  • Nanoparticles
  • Polysialic acid
  • Targeted cancer therapy

ASJC Scopus subject areas

  • Pharmaceutical Science

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