Hydroporator

A hydrodynamic cell membrane perforator for high-throughput vector-free nanomaterial intracellular delivery and DNA origami biostability evaluation

Megan E. Kizer, Yanxiang Deng, Geoumyoung Kang, Paiyz E. Mikael, Xing Wang, Aram Chung

Research output: Contribution to journalArticle

Abstract

The successful intracellular delivery of exogenous macromolecules is crucial for a variety of applications ranging from basic biology to the clinic. However, traditional intracellular delivery methods such as those relying on viral/non-viral nanocarriers or physical membrane disruptions suffer from low throughput, toxicity, and inconsistent delivery performance and are time-consuming and/or labor-intensive. In this study, we developed a single-step hydrodynamic cell deformation-induced intracellular delivery platform named "hydroporator" without the aid of vectors or a complicated/costly external apparatus. By utilizing only fluid inertia, the platform focuses, guides, and stretches cells robustly without clogging. This rapid hydrodynamic cell deformation leads to both convective and diffusive delivery of external (macro)molecules into the cell through transient plasma membrane discontinuities. Using this hydroporation approach, highly efficient (∼90%), high-throughput (>1600000 cells per min), and rapid delivery (∼1 min) of different (macro)molecules into a wide range of cell types was achieved while maintaining high cell viability. Taking advantage of the ability of this platform to rapidly deliver large molecules, we also systematically investigated the temporal biostability of vanilla DNA origami nanostructures in living cells for the first time. Experiments using two DNA origami (tube- and donut-shaped) nanostructures revealed that these nanostructures can maintain their structural integrity in living cells for approximately 1 h after delivery, providing new opportunities for the rapid characterization of intracellular DNA biostability.

Original languageEnglish
Pages (from-to)1747-1754
Number of pages8
JournalLab on a Chip
Volume19
Issue number10
DOIs
Publication statusPublished - 2019 Jan 1

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Perforators
Nanostructures
Hydrodynamics
Cell membranes
Nanostructured materials
DNA
Cells
Throughput
Cell Membrane
Molecules
Macros
Structural integrity
Macromolecules
Toxicity
Personnel
Membranes
Fluids
Vanilla
Experiments
Cell Survival

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Hydroporator : A hydrodynamic cell membrane perforator for high-throughput vector-free nanomaterial intracellular delivery and DNA origami biostability evaluation. / Kizer, Megan E.; Deng, Yanxiang; Kang, Geoumyoung; Mikael, Paiyz E.; Wang, Xing; Chung, Aram.

In: Lab on a Chip, Vol. 19, No. 10, 01.01.2019, p. 1747-1754.

Research output: Contribution to journalArticle

Kizer, Megan E. ; Deng, Yanxiang ; Kang, Geoumyoung ; Mikael, Paiyz E. ; Wang, Xing ; Chung, Aram. / Hydroporator : A hydrodynamic cell membrane perforator for high-throughput vector-free nanomaterial intracellular delivery and DNA origami biostability evaluation. In: Lab on a Chip. 2019 ; Vol. 19, No. 10. pp. 1747-1754.
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