Hypertonic saline downregulate the production level of lipopolysaccharide- induced migration inhibitory factor in THP-1 cells

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Macrophage migration inhibitory factor (MIF) may serve as a general marker for systemic inflammation in septic and nonseptic acute critical illness. Additionally, our previous experiment has demonstrated that immunosuppressant Prostaglandin E2 (PGE2) lowered MIF levels and inhibited T-cells proliferation when compared to control levels. The addition of hypertonic saline (HTS) increased MIF production as compared with PGE2-stimulated T-cells in concordance with restore PGE2-suppressed T-cells proliferation. Generally, HTS has been well known for its anti-inflammatory effect so far. Therefore, the experiments were conducted to evaluate MIF after stimulating lipopolysaccharide (LPS) either in the presence or absence of HTS in monocyte, in response to early phase injury. Methods: Human acute monocytic leukemic cell line (THP-1) cells were cultured in RPMI media, to a final concentration of 1 × 10 6 cells/mL. The effect of HTS on LPS-induced MIF was evaluated in monocyte with 1 μg/mL LPS. HTS at 10, 20 or 40 mmol/L above isotonicity was added. MIF concentrations of the supernatant were determined by enzyme-linked immunosorbent assay, and cell lysates were used for Western blots analysis to determine the MIF expression. Results: MIF concentrations in the cell supernatant increased in LPS-induced cells compared to control cells. Also, levels of MIF protein expression were higher in LPS stimulating cells. However, the addition of HTS to LPS stimulated cell restored MIF concentrations and MIF expression. Conclusion: The role of HTS in maintaining physiological balance in human beings, at least in part, should be mediated through the MIF pathway.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of the Korean Surgical Society
Volume82
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Lipopolysaccharides
Down-Regulation
Dinoprostone
T-Lymphocytes
Monocytes
Cell Proliferation
Macrophage Migration-Inhibitory Factors
Immunosuppressive Agents
Critical Illness
Cell Movement
Cultured Cells
Anti-Inflammatory Agents
Western Blotting
Enzyme-Linked Immunosorbent Assay
Inflammation
Cell Line
Wounds and Injuries
Proteins

Keywords

  • Anti-inflammatory agents
  • Hypertonic saline solution
  • Immunosuppression
  • Lipopolysaccharides
  • Macrophage migration-inhibitory factors

ASJC Scopus subject areas

  • Surgery

Cite this

@article{9d77b420e51548aa9f0832577b5ea45e,
title = "Hypertonic saline downregulate the production level of lipopolysaccharide- induced migration inhibitory factor in THP-1 cells",
abstract = "Purpose: Macrophage migration inhibitory factor (MIF) may serve as a general marker for systemic inflammation in septic and nonseptic acute critical illness. Additionally, our previous experiment has demonstrated that immunosuppressant Prostaglandin E2 (PGE2) lowered MIF levels and inhibited T-cells proliferation when compared to control levels. The addition of hypertonic saline (HTS) increased MIF production as compared with PGE2-stimulated T-cells in concordance with restore PGE2-suppressed T-cells proliferation. Generally, HTS has been well known for its anti-inflammatory effect so far. Therefore, the experiments were conducted to evaluate MIF after stimulating lipopolysaccharide (LPS) either in the presence or absence of HTS in monocyte, in response to early phase injury. Methods: Human acute monocytic leukemic cell line (THP-1) cells were cultured in RPMI media, to a final concentration of 1 × 10 6 cells/mL. The effect of HTS on LPS-induced MIF was evaluated in monocyte with 1 μg/mL LPS. HTS at 10, 20 or 40 mmol/L above isotonicity was added. MIF concentrations of the supernatant were determined by enzyme-linked immunosorbent assay, and cell lysates were used for Western blots analysis to determine the MIF expression. Results: MIF concentrations in the cell supernatant increased in LPS-induced cells compared to control cells. Also, levels of MIF protein expression were higher in LPS stimulating cells. However, the addition of HTS to LPS stimulated cell restored MIF concentrations and MIF expression. Conclusion: The role of HTS in maintaining physiological balance in human beings, at least in part, should be mediated through the MIF pathway.",
keywords = "Anti-inflammatory agents, Hypertonic saline solution, Immunosuppression, Lipopolysaccharides, Macrophage migration-inhibitory factors",
author = "Cheul Han and Choi, {Sung Hyuk} and Young-Hoon Yoon and Cho, {Young Duck} and Jung-Youn Kim and Hong, {Yun Sik} and Lee, {Sung Woo} and Sungwoo Moon and Cho, {Han Jin} and Cheon, {Young Jin}",
year = "2012",
month = "1",
day = "1",
doi = "10.4174/jkss.2012.82.1.1",
language = "English",
volume = "82",
pages = "1--7",
journal = "Annals of Surgical Treatment and Research",
issn = "2288-6575",
publisher = "Korean Surgical Society",
number = "1",

}

TY - JOUR

T1 - Hypertonic saline downregulate the production level of lipopolysaccharide- induced migration inhibitory factor in THP-1 cells

AU - Han, Cheul

AU - Choi, Sung Hyuk

AU - Yoon, Young-Hoon

AU - Cho, Young Duck

AU - Kim, Jung-Youn

AU - Hong, Yun Sik

AU - Lee, Sung Woo

AU - Moon, Sungwoo

AU - Cho, Han Jin

AU - Cheon, Young Jin

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Purpose: Macrophage migration inhibitory factor (MIF) may serve as a general marker for systemic inflammation in septic and nonseptic acute critical illness. Additionally, our previous experiment has demonstrated that immunosuppressant Prostaglandin E2 (PGE2) lowered MIF levels and inhibited T-cells proliferation when compared to control levels. The addition of hypertonic saline (HTS) increased MIF production as compared with PGE2-stimulated T-cells in concordance with restore PGE2-suppressed T-cells proliferation. Generally, HTS has been well known for its anti-inflammatory effect so far. Therefore, the experiments were conducted to evaluate MIF after stimulating lipopolysaccharide (LPS) either in the presence or absence of HTS in monocyte, in response to early phase injury. Methods: Human acute monocytic leukemic cell line (THP-1) cells were cultured in RPMI media, to a final concentration of 1 × 10 6 cells/mL. The effect of HTS on LPS-induced MIF was evaluated in monocyte with 1 μg/mL LPS. HTS at 10, 20 or 40 mmol/L above isotonicity was added. MIF concentrations of the supernatant were determined by enzyme-linked immunosorbent assay, and cell lysates were used for Western blots analysis to determine the MIF expression. Results: MIF concentrations in the cell supernatant increased in LPS-induced cells compared to control cells. Also, levels of MIF protein expression were higher in LPS stimulating cells. However, the addition of HTS to LPS stimulated cell restored MIF concentrations and MIF expression. Conclusion: The role of HTS in maintaining physiological balance in human beings, at least in part, should be mediated through the MIF pathway.

AB - Purpose: Macrophage migration inhibitory factor (MIF) may serve as a general marker for systemic inflammation in septic and nonseptic acute critical illness. Additionally, our previous experiment has demonstrated that immunosuppressant Prostaglandin E2 (PGE2) lowered MIF levels and inhibited T-cells proliferation when compared to control levels. The addition of hypertonic saline (HTS) increased MIF production as compared with PGE2-stimulated T-cells in concordance with restore PGE2-suppressed T-cells proliferation. Generally, HTS has been well known for its anti-inflammatory effect so far. Therefore, the experiments were conducted to evaluate MIF after stimulating lipopolysaccharide (LPS) either in the presence or absence of HTS in monocyte, in response to early phase injury. Methods: Human acute monocytic leukemic cell line (THP-1) cells were cultured in RPMI media, to a final concentration of 1 × 10 6 cells/mL. The effect of HTS on LPS-induced MIF was evaluated in monocyte with 1 μg/mL LPS. HTS at 10, 20 or 40 mmol/L above isotonicity was added. MIF concentrations of the supernatant were determined by enzyme-linked immunosorbent assay, and cell lysates were used for Western blots analysis to determine the MIF expression. Results: MIF concentrations in the cell supernatant increased in LPS-induced cells compared to control cells. Also, levels of MIF protein expression were higher in LPS stimulating cells. However, the addition of HTS to LPS stimulated cell restored MIF concentrations and MIF expression. Conclusion: The role of HTS in maintaining physiological balance in human beings, at least in part, should be mediated through the MIF pathway.

KW - Anti-inflammatory agents

KW - Hypertonic saline solution

KW - Immunosuppression

KW - Lipopolysaccharides

KW - Macrophage migration-inhibitory factors

UR - http://www.scopus.com/inward/record.url?scp=84863047245&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863047245&partnerID=8YFLogxK

U2 - 10.4174/jkss.2012.82.1.1

DO - 10.4174/jkss.2012.82.1.1

M3 - Article

C2 - 22324039

AN - SCOPUS:84863047245

VL - 82

SP - 1

EP - 7

JO - Annals of Surgical Treatment and Research

JF - Annals of Surgical Treatment and Research

SN - 2288-6575

IS - 1

ER -