Hypocholesterolemic and hypoglycemic effects of enzymatically modified carbohydrates from rice in high-fat-fed C57BL/6J mice

Muhammad J H Bhuiyan, Ha Viet Do, Saehun Mun, Hee jin Jun, Ji Hae Lee, Yong Ro Kim, Sung-Joon Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Scope: Enzymatically modified rice starch (ERS) synthesized with 4-α-glucanotransferase has a longer structure than rice starch, which could delay digestion, similar to dietary fiber. We investigated the effects of ERS on glucose and lipid metabolism with mice fed a high-fat diet containing ERS (HFD-ERS). Method and results: Four weeks of ERS feeding showed hypoglycemic effects with a significant reduction in fasting glucose (46%), insulin (57%), and leptin (83%) levels; improved glucose tolerance (20% in AUC of oral glucose tolerance test); and increased adiponectin concentrations (+27%) compared to the HFD group. Notably, phosphorylation of AMP kinase (AMPK) was markedly induced in the HFD-ERS livers compared to HFD livers. Additionally, ERS significantly reduced total cholesterol concentrations with induction of fecal bile acid excretion (+21%, P<0.05) in the HFD-ERS group compared to the HFD group. The mRNA and protein expressions of hepatic LDL receptors were significantly induced. However, cholesterol 7 alpha-hydroxylase (CYP7A1) expression was downregulated possibly due to induction of intestinal farnesoid X receptor (FXR; +2.4-fold, p<0.05) and fibroblast growth factor-15 (FGF-15; +2.2-fold, p<0.01). Conclusion: Our data suggest that ERS feeding may have hypoglycemic and hypocholesterolemic effects via a mechanism similar to that of dietary fiber.

Original languageEnglish
JournalMolecular Nutrition and Food Research
Volume55
Issue numberSUPPL. 2
DOIs
Publication statusPublished - 2011 Sep 1

Fingerprint

glycemic effect
rice starch
cholesteremic effect
Inbred C57BL Mouse
Hypoglycemic Agents
Starch
Fats
Carbohydrates
carbohydrates
rice
mice
lipids
High Fat Diet
high fat diet
Dietary Fiber
Glucose
liver
Liver
dietary fiber
cholesterol

Keywords

  • Bile acid excretion
  • Cholesterol
  • Glucose metabolism
  • Lipid metabolism
  • Rice starch

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Hypocholesterolemic and hypoglycemic effects of enzymatically modified carbohydrates from rice in high-fat-fed C57BL/6J mice. / Bhuiyan, Muhammad J H; Do, Ha Viet; Mun, Saehun; Jun, Hee jin; Lee, Ji Hae; Kim, Yong Ro; Lee, Sung-Joon.

In: Molecular Nutrition and Food Research, Vol. 55, No. SUPPL. 2, 01.09.2011.

Research output: Contribution to journalArticle

Bhuiyan, Muhammad J H ; Do, Ha Viet ; Mun, Saehun ; Jun, Hee jin ; Lee, Ji Hae ; Kim, Yong Ro ; Lee, Sung-Joon. / Hypocholesterolemic and hypoglycemic effects of enzymatically modified carbohydrates from rice in high-fat-fed C57BL/6J mice. In: Molecular Nutrition and Food Research. 2011 ; Vol. 55, No. SUPPL. 2.
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AU - Lee, Ji Hae

AU - Kim, Yong Ro

AU - Lee, Sung-Joon

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N2 - Scope: Enzymatically modified rice starch (ERS) synthesized with 4-α-glucanotransferase has a longer structure than rice starch, which could delay digestion, similar to dietary fiber. We investigated the effects of ERS on glucose and lipid metabolism with mice fed a high-fat diet containing ERS (HFD-ERS). Method and results: Four weeks of ERS feeding showed hypoglycemic effects with a significant reduction in fasting glucose (46%), insulin (57%), and leptin (83%) levels; improved glucose tolerance (20% in AUC of oral glucose tolerance test); and increased adiponectin concentrations (+27%) compared to the HFD group. Notably, phosphorylation of AMP kinase (AMPK) was markedly induced in the HFD-ERS livers compared to HFD livers. Additionally, ERS significantly reduced total cholesterol concentrations with induction of fecal bile acid excretion (+21%, P<0.05) in the HFD-ERS group compared to the HFD group. The mRNA and protein expressions of hepatic LDL receptors were significantly induced. However, cholesterol 7 alpha-hydroxylase (CYP7A1) expression was downregulated possibly due to induction of intestinal farnesoid X receptor (FXR; +2.4-fold, p<0.05) and fibroblast growth factor-15 (FGF-15; +2.2-fold, p<0.01). Conclusion: Our data suggest that ERS feeding may have hypoglycemic and hypocholesterolemic effects via a mechanism similar to that of dietary fiber.

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