Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats

Min-Jeong Shin, Yoonsu Cho, Jiyoung Moon, Hyun Ju Jeon, Seung Min Lee, Ji Hyung Chung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalFood and Chemical Toxicology
Volume57
DOIs
Publication statusPublished - 2013 Jul 1

Fingerprint

cholesteremic effect
Sprague Dawley Rats
Rats
Fats
Cholesterol
cholesterol
Nutrition
rats
lipids
high fat diet
liver
High Fat Diet
Metabolism
gene expression
cholesterol metabolism
Liver
hypercholesterolemia
Genes
bile acids
low density lipoprotein cholesterol

Keywords

  • Cholesterol
  • CYP7A1
  • Fisetin
  • HMG-CoA reductase
  • Hypercholesterolemia

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats. / Shin, Min-Jeong; Cho, Yoonsu; Moon, Jiyoung; Jeon, Hyun Ju; Lee, Seung Min; Chung, Ji Hyung.

In: Food and Chemical Toxicology, Vol. 57, 01.07.2013, p. 84-90.

Research output: Contribution to journalArticle

Shin, Min-Jeong ; Cho, Yoonsu ; Moon, Jiyoung ; Jeon, Hyun Ju ; Lee, Seung Min ; Chung, Ji Hyung. / Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats. In: Food and Chemical Toxicology. 2013 ; Vol. 57. pp. 84-90.
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