Abstract
We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.
| Original language | English |
|---|---|
| Pages (from-to) | 84-90 |
| Number of pages | 7 |
| Journal | Food and Chemical Toxicology |
| Volume | 57 |
| DOIs | |
| Publication status | Published - 2013 Jul 1 |
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Keywords
- Cholesterol
- CYP7A1
- Fisetin
- HMG-CoA reductase
- Hypercholesterolemia
ASJC Scopus subject areas
- Food Science
- Toxicology
Cite this
Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats. / Shin, Min-Jeong; Cho, Yoonsu; Moon, Jiyoung; Jeon, Hyun Ju; Lee, Seung Min; Chung, Ji Hyung.
In: Food and Chemical Toxicology, Vol. 57, 01.07.2013, p. 84-90.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats
AU - Shin, Min-Jeong
AU - Cho, Yoonsu
AU - Moon, Jiyoung
AU - Jeon, Hyun Ju
AU - Lee, Seung Min
AU - Chung, Ji Hyung
PY - 2013/7/1
Y1 - 2013/7/1
N2 - We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.
AB - We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.
KW - Cholesterol
KW - CYP7A1
KW - Fisetin
KW - HMG-CoA reductase
KW - Hypercholesterolemia
UR - http://www.scopus.com/inward/record.url?scp=84876310011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876310011&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2013.03.010
DO - 10.1016/j.fct.2013.03.010
M3 - Article
VL - 57
SP - 84
EP - 90
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
SN - 0278-6915
ER -