Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats

Min Jeong Shin; Yoonsu Cho; Jiyoung Moon; Hyun Ju Jeon; Seung Min Lee; Ji Hyung Chung

doi:10.1016/j.fct.2013.03.010

Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats

Min Jeong Shin, Yoonsu Cho, Jiyoung Moon, Hyun Ju Jeon, Seung Min Lee, Ji Hyung Chung

Department of Public Health Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.

Original language	English
Pages (from-to)	84-90
Number of pages	7
Journal	Food and Chemical Toxicology
Volume	57
DOIs	https://doi.org/10.1016/j.fct.2013.03.010
Publication status	Published - 2013 Jul

Keywords

CYP7A1
Cholesterol
Fisetin
HMG-CoA reductase
Hypercholesterolemia

ASJC Scopus subject areas

Food Science
Toxicology

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10.1016/j.fct.2013.03.010

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title = "Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats",

abstract = "We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.",

keywords = "CYP7A1, Cholesterol, Fisetin, HMG-CoA reductase, Hypercholesterolemia",

author = "Shin, {Min Jeong} and Yoonsu Cho and Jiyoung Moon and Jeon, {Hyun Ju} and Lee, {Seung Min} and Chung, {Ji Hyung}",

note = "Funding Information: This research was supported by Basic Science Research Program through the National research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0002119). ",

year = "2013",

month = jul,

doi = "10.1016/j.fct.2013.03.010",

language = "English",

volume = "57",

pages = "84--90",

journal = "Food and Chemical Toxicology",

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T1 - Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats

AU - Shin, Min Jeong

AU - Cho, Yoonsu

AU - Moon, Jiyoung

AU - Jeon, Hyun Ju

AU - Lee, Seung Min

AU - Chung, Ji Hyung

N1 - Funding Information: This research was supported by Basic Science Research Program through the National research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0002119).

PY - 2013/7

Y1 - 2013/7

N2 - We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.

AB - We aimed to test whether fisetin could modulate cholesterol homeostasis in rats with diet-induced hypercholesterolemia, and further investigated the underlying mechanisms by which fisetin exerts its cholesterol lowering effect. Blood lipid profile, hepatic cholesterol content, as well as gene expressions in cholesterol metabolism were examined. Elevated levels of total cholesterol and LDL-cholesterol, along with hepatic cholesterol content in a high fat group were found to be significantly reduced by fisetin. The high fat diet significantly decreased hepatic mRNA levels of LDLR, SREBP2, HMGCR and PCSK9 in comparison to the control diet, however, fisetin did not further elicit any changes in mRNA levels of the same genes. The high fat diet dramatically increased the transcript levels of CYP7A1, which was subsequently reversed by the fisetin. In HepG2 cells, fisetin was found to increase the levels of a nuclear form of SREBP2 and LDLR. In conclusion, fisetin supplementation displayed hypocholesterolemic effects by modulating the expression of genes associated with cholesterol and bile acid metabolism.

KW - CYP7A1

KW - Cholesterol

KW - Fisetin

KW - HMG-CoA reductase

KW - Hypercholesterolemia

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Hypocholesterolemic effect of daily fisetin supplementation in high fat fed Sprague-Dawley rats

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