Hypocholesterolemic effects of the cauliflower culinary-medicinal mushroom, sparassis crispa (Higher basidiomycetes), in diet-induced hypercholesterolemic rats

Ki Bae Hong, Sung Yong Hong, Eun Young Joung, Byung Hee Kim, Song Hwan Bae, Yooheon Park, Hyung Joo Suh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The cauliflower culinary-medicinal mushroom, Sparassis crispa, possesses various biological activities that have been widely reported to have therapeutic applications. We examined the effects of S. crispa on serum cholesterol, hepatic enzymes related to cholesterol metabolism, and fecal sterol excretion in rats fed a cholesterolrich diet for 4 weeks. Male Sprague-Dawley rats (8 weeks old) were randomly divided into 5 groups (n = 6 mice per group): normal diet (normal control [NC]), cholesterol-rich diet (cholesterol control [CC]), cholesterol-rich diet plus S. crispa fruiting body (SC), cholesterol-rich diet plus S. crispa extract (SCE), and cholesterol-rich diet plus S. crispa residue (SCR). SCE supplementation significantly enhanced hepatic cholesterol catabolism through the upregulation of cholesterol 7α-hydroxylase (CYP7A1) messenger RNA (mRNA) expression (2.55-fold compared with that in the NC group; P <0.05) and the downregulation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase mRNA expression (0.57-fold compared with that in the NC group; P <0.05). Additionally, the SCE diet resulted in the highest fecal excretion of cholesterol and bile acid in hypercholesterolemic rats. In conclusion, mRNA expression of CYP7A1 and HMG-CoA reductase were significantly modulated by the absorption of SCE samples. Also, SCE samples had a significant effect on fecal bile acid and cholesterol excretion. These results suggest that SCE samples can induce hypocholesterolic effects through cholesterol metabolism and the reduction of circulating cholesterol levels.

Original languageEnglish
Pages (from-to)965-975
Number of pages11
JournalInternational Journal of Medicinal Mushrooms
Volume17
Issue number10
Publication statusPublished - 2015

Fingerprint

Basidiomycota
Agaricales
Brassica
Cholesterol
Diet
Bile Acids and Salts
Messenger RNA
Oxidoreductases
Cholesterol 7-alpha-Hydroxylase
Control Groups
Liver
Sterols
Coenzyme A
Sprague Dawley Rats
Up-Regulation
Down-Regulation

Keywords

  • 3-hydroxy-3-methylglutaryl coenzyme A
  • Cholesterol
  • Cholesterol 7α-hydroxylase
  • Hypercholesterolemia
  • Medicinal mushrooms
  • Sparassis crispa

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Pharmacology

Cite this

Hypocholesterolemic effects of the cauliflower culinary-medicinal mushroom, sparassis crispa (Higher basidiomycetes), in diet-induced hypercholesterolemic rats. / Hong, Ki Bae; Hong, Sung Yong; Joung, Eun Young; Kim, Byung Hee; Bae, Song Hwan; Park, Yooheon; Suh, Hyung Joo.

In: International Journal of Medicinal Mushrooms, Vol. 17, No. 10, 2015, p. 965-975.

Research output: Contribution to journalArticle

Hong, Ki Bae ; Hong, Sung Yong ; Joung, Eun Young ; Kim, Byung Hee ; Bae, Song Hwan ; Park, Yooheon ; Suh, Hyung Joo. / Hypocholesterolemic effects of the cauliflower culinary-medicinal mushroom, sparassis crispa (Higher basidiomycetes), in diet-induced hypercholesterolemic rats. In: International Journal of Medicinal Mushrooms. 2015 ; Vol. 17, No. 10. pp. 965-975.
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abstract = "The cauliflower culinary-medicinal mushroom, Sparassis crispa, possesses various biological activities that have been widely reported to have therapeutic applications. We examined the effects of S. crispa on serum cholesterol, hepatic enzymes related to cholesterol metabolism, and fecal sterol excretion in rats fed a cholesterolrich diet for 4 weeks. Male Sprague-Dawley rats (8 weeks old) were randomly divided into 5 groups (n = 6 mice per group): normal diet (normal control [NC]), cholesterol-rich diet (cholesterol control [CC]), cholesterol-rich diet plus S. crispa fruiting body (SC), cholesterol-rich diet plus S. crispa extract (SCE), and cholesterol-rich diet plus S. crispa residue (SCR). SCE supplementation significantly enhanced hepatic cholesterol catabolism through the upregulation of cholesterol 7α-hydroxylase (CYP7A1) messenger RNA (mRNA) expression (2.55-fold compared with that in the NC group; P <0.05) and the downregulation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase mRNA expression (0.57-fold compared with that in the NC group; P <0.05). Additionally, the SCE diet resulted in the highest fecal excretion of cholesterol and bile acid in hypercholesterolemic rats. In conclusion, mRNA expression of CYP7A1 and HMG-CoA reductase were significantly modulated by the absorption of SCE samples. Also, SCE samples had a significant effect on fecal bile acid and cholesterol excretion. These results suggest that SCE samples can induce hypocholesterolic effects through cholesterol metabolism and the reduction of circulating cholesterol levels.",
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AU - Hong, Sung Yong

AU - Joung, Eun Young

AU - Kim, Byung Hee

AU - Bae, Song Hwan

AU - Park, Yooheon

AU - Suh, Hyung Joo

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