Hypoglycaemic effects of functional tri-peptides from silk in differentiated adipocytes and streptozotocin-induced diabetic mice

Bok Kyung Han, Hyun Jung Lee, Hyun Sun Lee, Hyung Joo Suh, Yooheon Park

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

BACKGROUND: In this study, the tri-peptides Gly-Glu-Tyr (GEY) and Gly-Tyr-Gly (GYG), identified previously as active compounds from the silk peptide E5K6, significantly stimulated basal and insulin-mediated glucose uptake by 3T3-L1 fibroblasts in a dose-dependent manner. RESULTS: Synthetic GEY and GYG peptides at a concentration of 500 μmol L-1 significantly increased glucose transporter type 4 expression by 157% and 239%, respectively. Differentiation of 3T3-L1 cells into adipocytes leads to accumulation of intracellular fat droplets, and GEY and GYG at a concentration of 250 μmol L-1 suppressed this effect by 72% and 75%, respectively. GYG improved glucose tolerance in steptozotocin (STZ)-induced diabetic mice in a dose-dependent manner. CONCLUSION: These results suggest that GYG isolated from E5K6 has anti-diabetic potential and silk waste products containing bioactive peptides could be used to the developments of treatments to lower blood glucose.

Original languageEnglish
Pages (from-to)116-121
Number of pages6
JournalJournal of the Science of Food and Agriculture
Volume96
Issue number1
DOIs
Publication statusPublished - 2016 Jan 15

Fingerprint

glycyl-tyrosyl-glycine
glycemic effect
Silk
streptozotocin
Streptozocin
silk
adipocytes
Hypoglycemic Agents
Adipocytes
peptides
Peptides
mice
glucose transporters
Glucose Transporter Type 4
3T3-L1 Cells
Waste Products
glucose tolerance
Glucose
dosage
blood glucose

Keywords

  • 3T3-L1 adipocyte
  • Alcalase
  • Glucose uptake
  • Silk peptide E5K6
  • STZ-induced diabetic mice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Nutrition and Dietetics
  • Biotechnology

Cite this

Hypoglycaemic effects of functional tri-peptides from silk in differentiated adipocytes and streptozotocin-induced diabetic mice. / Han, Bok Kyung; Lee, Hyun Jung; Lee, Hyun Sun; Suh, Hyung Joo; Park, Yooheon.

In: Journal of the Science of Food and Agriculture, Vol. 96, No. 1, 15.01.2016, p. 116-121.

Research output: Contribution to journalArticle

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AU - Park, Yooheon

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AB - BACKGROUND: In this study, the tri-peptides Gly-Glu-Tyr (GEY) and Gly-Tyr-Gly (GYG), identified previously as active compounds from the silk peptide E5K6, significantly stimulated basal and insulin-mediated glucose uptake by 3T3-L1 fibroblasts in a dose-dependent manner. RESULTS: Synthetic GEY and GYG peptides at a concentration of 500 μmol L-1 significantly increased glucose transporter type 4 expression by 157% and 239%, respectively. Differentiation of 3T3-L1 cells into adipocytes leads to accumulation of intracellular fat droplets, and GEY and GYG at a concentration of 250 μmol L-1 suppressed this effect by 72% and 75%, respectively. GYG improved glucose tolerance in steptozotocin (STZ)-induced diabetic mice in a dose-dependent manner. CONCLUSION: These results suggest that GYG isolated from E5K6 has anti-diabetic potential and silk waste products containing bioactive peptides could be used to the developments of treatments to lower blood glucose.

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