Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress

Mi Seon Shin, Hyukki Chang, Churl Namkoong, Gil Myoung Kang, Hyun Kyong Kim, So Young Gil, Ji Hee Yu, Kyeong Han Park, Min Seon Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Clusterin is a sulfated glycoprotein abundantly expressed in the pituitary gland and hypothalamus of mammals. However, its physiological role in neuroendocrine function is largely unknown. In the present study, we investigated the effects of intracerebroventricular (ICV) administration of clusterin on plasma pituitary hormone levels in normal rats. Single ICV injection of clusterin provoked neurohormonal changes seen under acute stress condition: increased plasma adrenocorticotropic hormone (ACTH), corticosterone, GH and prolactin levels and decreased LH and FSH levels. Consistently, hypothalamic and pituitary clusterin expression levels were upregulated following a restraint stress, suggesting an involvement of endogenous clusterin in stress-induced neurohormonal changes. In the pituitary intermediate lobe, clusterin was coexpressed with proopiomelanocortin (POMC), a precursor of ACTH. Treatment of clusterin in POMC expressing AtT-20 pituitary cells increased basal and corticotropin-releasing hormone (CRH)-stimulated POMC promoter activities and intracellular cAMP levels. Furthermore, clusterin treatment triggered ACTH secretion from AtT-20 cells in a CRH-dependent manner, indicating that increased clusterin under stressful conditions may augment CRH-stimulated ACTH production and release. In summary, hypothalamic and pituitary clusterin may function as a modulator of neurohormonal responses under stressful conditions.

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalNeuroendocrinology
Volume98
Issue number3
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

Clusterin
Pro-Opiomelanocortin
Adrenocorticotropic Hormone
Corticotropin-Releasing Hormone
Intermediate Pituitary Gland
Pituitary Hormones
Pituitary Gland
Corticosterone
Prolactin
Hypothalamus
Mammals
Glycoproteins

Keywords

  • Clusterin
  • Corticotropin
  • Neurohormonal changes
  • Proopiomelanocortin
  • Stress

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Shin, M. S., Chang, H., Namkoong, C., Kang, G. M., Kim, H. K., Gil, S. Y., ... Kim, M. S. (2013). Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress. Neuroendocrinology, 98(3), 233-241. https://doi.org/10.1159/000355625

Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress. / Shin, Mi Seon; Chang, Hyukki; Namkoong, Churl; Kang, Gil Myoung; Kim, Hyun Kyong; Gil, So Young; Yu, Ji Hee; Park, Kyeong Han; Kim, Min Seon.

In: Neuroendocrinology, Vol. 98, No. 3, 01.01.2013, p. 233-241.

Research output: Contribution to journalArticle

Shin, MS, Chang, H, Namkoong, C, Kang, GM, Kim, HK, Gil, SY, Yu, JH, Park, KH & Kim, MS 2013, 'Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress', Neuroendocrinology, vol. 98, no. 3, pp. 233-241. https://doi.org/10.1159/000355625
Shin MS, Chang H, Namkoong C, Kang GM, Kim HK, Gil SY et al. Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress. Neuroendocrinology. 2013 Jan 1;98(3):233-241. https://doi.org/10.1159/000355625
Shin, Mi Seon ; Chang, Hyukki ; Namkoong, Churl ; Kang, Gil Myoung ; Kim, Hyun Kyong ; Gil, So Young ; Yu, Ji Hee ; Park, Kyeong Han ; Kim, Min Seon. / Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress. In: Neuroendocrinology. 2013 ; Vol. 98, No. 3. pp. 233-241.
@article{16ac9e4868164b64b05e4744efb6d686,
title = "Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress",
abstract = "Clusterin is a sulfated glycoprotein abundantly expressed in the pituitary gland and hypothalamus of mammals. However, its physiological role in neuroendocrine function is largely unknown. In the present study, we investigated the effects of intracerebroventricular (ICV) administration of clusterin on plasma pituitary hormone levels in normal rats. Single ICV injection of clusterin provoked neurohormonal changes seen under acute stress condition: increased plasma adrenocorticotropic hormone (ACTH), corticosterone, GH and prolactin levels and decreased LH and FSH levels. Consistently, hypothalamic and pituitary clusterin expression levels were upregulated following a restraint stress, suggesting an involvement of endogenous clusterin in stress-induced neurohormonal changes. In the pituitary intermediate lobe, clusterin was coexpressed with proopiomelanocortin (POMC), a precursor of ACTH. Treatment of clusterin in POMC expressing AtT-20 pituitary cells increased basal and corticotropin-releasing hormone (CRH)-stimulated POMC promoter activities and intracellular cAMP levels. Furthermore, clusterin treatment triggered ACTH secretion from AtT-20 cells in a CRH-dependent manner, indicating that increased clusterin under stressful conditions may augment CRH-stimulated ACTH production and release. In summary, hypothalamic and pituitary clusterin may function as a modulator of neurohormonal responses under stressful conditions.",
keywords = "Clusterin, Corticotropin, Neurohormonal changes, Proopiomelanocortin, Stress",
author = "Shin, {Mi Seon} and Hyukki Chang and Churl Namkoong and Kang, {Gil Myoung} and Kim, {Hyun Kyong} and Gil, {So Young} and Yu, {Ji Hee} and Park, {Kyeong Han} and Kim, {Min Seon}",
year = "2013",
month = "1",
day = "1",
doi = "10.1159/000355625",
language = "English",
volume = "98",
pages = "233--241",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Hypothalamic and pituitary clusterin modulates neurohormonal responses to stress

AU - Shin, Mi Seon

AU - Chang, Hyukki

AU - Namkoong, Churl

AU - Kang, Gil Myoung

AU - Kim, Hyun Kyong

AU - Gil, So Young

AU - Yu, Ji Hee

AU - Park, Kyeong Han

AU - Kim, Min Seon

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Clusterin is a sulfated glycoprotein abundantly expressed in the pituitary gland and hypothalamus of mammals. However, its physiological role in neuroendocrine function is largely unknown. In the present study, we investigated the effects of intracerebroventricular (ICV) administration of clusterin on plasma pituitary hormone levels in normal rats. Single ICV injection of clusterin provoked neurohormonal changes seen under acute stress condition: increased plasma adrenocorticotropic hormone (ACTH), corticosterone, GH and prolactin levels and decreased LH and FSH levels. Consistently, hypothalamic and pituitary clusterin expression levels were upregulated following a restraint stress, suggesting an involvement of endogenous clusterin in stress-induced neurohormonal changes. In the pituitary intermediate lobe, clusterin was coexpressed with proopiomelanocortin (POMC), a precursor of ACTH. Treatment of clusterin in POMC expressing AtT-20 pituitary cells increased basal and corticotropin-releasing hormone (CRH)-stimulated POMC promoter activities and intracellular cAMP levels. Furthermore, clusterin treatment triggered ACTH secretion from AtT-20 cells in a CRH-dependent manner, indicating that increased clusterin under stressful conditions may augment CRH-stimulated ACTH production and release. In summary, hypothalamic and pituitary clusterin may function as a modulator of neurohormonal responses under stressful conditions.

AB - Clusterin is a sulfated glycoprotein abundantly expressed in the pituitary gland and hypothalamus of mammals. However, its physiological role in neuroendocrine function is largely unknown. In the present study, we investigated the effects of intracerebroventricular (ICV) administration of clusterin on plasma pituitary hormone levels in normal rats. Single ICV injection of clusterin provoked neurohormonal changes seen under acute stress condition: increased plasma adrenocorticotropic hormone (ACTH), corticosterone, GH and prolactin levels and decreased LH and FSH levels. Consistently, hypothalamic and pituitary clusterin expression levels were upregulated following a restraint stress, suggesting an involvement of endogenous clusterin in stress-induced neurohormonal changes. In the pituitary intermediate lobe, clusterin was coexpressed with proopiomelanocortin (POMC), a precursor of ACTH. Treatment of clusterin in POMC expressing AtT-20 pituitary cells increased basal and corticotropin-releasing hormone (CRH)-stimulated POMC promoter activities and intracellular cAMP levels. Furthermore, clusterin treatment triggered ACTH secretion from AtT-20 cells in a CRH-dependent manner, indicating that increased clusterin under stressful conditions may augment CRH-stimulated ACTH production and release. In summary, hypothalamic and pituitary clusterin may function as a modulator of neurohormonal responses under stressful conditions.

KW - Clusterin

KW - Corticotropin

KW - Neurohormonal changes

KW - Proopiomelanocortin

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=84898446236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898446236&partnerID=8YFLogxK

U2 - 10.1159/000355625

DO - 10.1159/000355625

M3 - Article

C2 - 24080898

AN - SCOPUS:84898446236

VL - 98

SP - 233

EP - 241

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 3

ER -