ID4 imparts chemoresistance and cancer stemness to glioma cells by derepressing miR-9*-mediated suppression of SOX2

Hye Min Jeon, Young Woo Sohn, Se Young Oh, Sung Hak Kim, Samuel Beck, Soonhag Kim, Hyunggee Kim

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Glioma stem cells (GSC) possess tumor-initiating potential and are relatively resistant to conventional chemotherapy and irradiation. Thus, they are considered to be major drivers for glioma initiation, progression, and recurrence. However, the precise mechanism governing acquisition of their drug resistance remains to be elucidated. Our previous study has shown that inhibitor of differentiation 4 (ID4) dedifferentiates Ink4a/Arf-/- mouse astrocytes and human glioma cells to glioma stem-like cells (induced GSCs or iGSCs). In this article, we report that ID4-driven iGSCs exhibit chemoresistant behavior to anticancer drugs through activation of ATPbinding cassette (ABC) transporters. We found that ID4 enhanced SOX2 protein expression by suppressing microRNA-9* (miR-9*), which can repress SOX2 by targeting its 3′-untranslated region. Consequently, ID4-mediated SOX2 induction enhanced ABCC3 and ABCC6 expression through direct transcriptional regulation, indicating that ID4 regulates the chemoresistance of iGSCs by promoting SOX2-mediated induction of ABC transporters. Furthermore, we found that short hairpin RNA-mediated knockdown of SOX2 in ID4-driven iGSCs resulted in loss of cancer stemness. Moreover, ectopic expression of SOX2 could dedifferentiate Ink4a/Arf-/- astrocytes and glioma cells to iGSCs, indicating a crucial role of SOX2 in genesis and maintenance of GSCs. Finally, we found that the significance of the ID4-miR-9*-SOX2-ABCC3/ABCC6 regulatory pathway is recapitulated in GSCs derived from patients with glioma. Together, our results reveal a novel regulatory mechanism by which ID4-driven suppression of miR-9* induces SOX2, which imparts stemness potential and chemoresistance to glioma cells and GSCs.

Original languageEnglish
Pages (from-to)3410-3421
Number of pages12
JournalCancer Research
Volume71
Issue number9
DOIs
Publication statusPublished - 2011 May 1

Fingerprint

MicroRNAs
Glioma
Neoplasms
Neoplastic Stem Cells
Astrocytes
3' Untranslated Regions
Drug Resistance
Small Interfering RNA
Stem Cells
Maintenance
Recurrence
Drug Therapy
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

ID4 imparts chemoresistance and cancer stemness to glioma cells by derepressing miR-9*-mediated suppression of SOX2. / Jeon, Hye Min; Sohn, Young Woo; Oh, Se Young; Kim, Sung Hak; Beck, Samuel; Kim, Soonhag; Kim, Hyunggee.

In: Cancer Research, Vol. 71, No. 9, 01.05.2011, p. 3410-3421.

Research output: Contribution to journalArticle

Jeon, Hye Min ; Sohn, Young Woo ; Oh, Se Young ; Kim, Sung Hak ; Beck, Samuel ; Kim, Soonhag ; Kim, Hyunggee. / ID4 imparts chemoresistance and cancer stemness to glioma cells by derepressing miR-9*-mediated suppression of SOX2. In: Cancer Research. 2011 ; Vol. 71, No. 9. pp. 3410-3421.
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