Identification and biocontrol efficacy of Streptomyces miharaensis producing filipin III against Fusarium wilt

Jeong Do Kim, Jae Woo Han, In Cheon Hwang, Dongho Lee, Beom Seok Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A number of bacterial strains were isolated from the internal tissue of Trapa japonica. Of these, strain KPE62302H, which had a 16S rDNA sequence identical to that of Streptomyces miharaensis showed antifungal activity against several plant pathogens. Treatment of seeds with strain KPE62302H induced a significant reduction in the incidence of Fusarium wilt in tomato plants compared with untreated controls. An antifungal substance (FP-1) was purified from the culture extract of strain KPE62302H using C18 flash and Sephadex LH-20 column chromatography and reverse phase HPLC. Extensive spectrometric analysis using MS and NMR identified this as filipin III. FP-1 inhibited the mycelial growth of plant pathogenic fungi such as Alternaria mali, Aspergillus niger, Colletotrichum gloeosporioides, C. orbiculare, Cylindrocarpon destructans, Diaporthe citiri, Fusarium oxysporum at 1-10 μg ml -1 and also markedly inhibited the development of Fusarium wilt caused by F. oxysporum f.sp. lycopersici in tomato plants by treatment with 10 μg ml -1 under greenhouse conditions. The efficacy of FP-1 against Fusarium wilt was comparable to that of the synthetic fungicide benomyl. An egfp -tagged strain of KPE62302H confirmed its ability to colonize tomato plants.

Original languageEnglish
Pages (from-to)150-159
Number of pages10
JournalJournal of Basic Microbiology
Volume52
Issue number2
DOIs
Publication statusPublished - 2012 Apr

Keywords

  • Antagonistic bacteria
  • Biofungicides
  • Filipin III
  • Fusarium wilt
  • Streptomyces miharaensis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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