TY - JOUR
T1 - Identification and characterization of a human cDNA homologous to yeast SKI2
AU - Lee, Seong Gene
AU - Lee, Inchul
AU - Park, Sun H.
AU - Kang, Changwon
AU - Song, Kyuyoung
N1 - Funding Information:
We thank Yeonhee Choi and Jueun Chung for excellent technical assistance, Bernice Morrow for the earlier database search, and Se-yeon Weon for database assistance. We are grateful to Drs. Raju Kucherlapati, Oliver Smithies, Nobuyo Maeda, and Kyunghee Choi for helpful discussions. This work was supported by grants from the Asan Foundation through the Asan Institute for Life Sciences to K.S. and I.L. and Grant 913-0402-019-2 from the Korean Science and Engineering Foundation to IL.
PY - 1995
Y1 - 1995
N2 - A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SKI2. The SKI2 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21.
AB - A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SKI2. The SKI2 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21.
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U2 - 10.1016/0888-7543(95)80008-A
DO - 10.1016/0888-7543(95)80008-A
M3 - Article
C2 - 7759100
AN - SCOPUS:0028922185
VL - 25
SP - 660
EP - 666
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -