Identification and characterization of a human cDNA homologous to yeast SKI2

Seong Gene Lee; Inchul Lee; Sun H. Park; Changwon Kang; Kyuyoung Song

doi:10.1016/0888-7543(95)80008-A

Identification and characterization of a human cDNA homologous to yeast SKI2

Seong Gene Lee, Inchul Lee, Sun H. Park, Changwon Kang, Kyuyoung Song

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SKI2. The SKI2 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21.

Original language	English
Pages (from-to)	660-666
Number of pages	7
Journal	Genomics
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/0888-7543(95)80008-A
Publication status	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Genetics

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10.1016/0888-7543(95)80008-A

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@article{b438aa8fb72a4d25be9dedef3e0b3afd,

title = "Identification and characterization of a human cDNA homologous to yeast SKI2",

abstract = "A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SKI2. The SKI2 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21.",

author = "Lee, {Seong Gene} and Inchul Lee and Park, {Sun H.} and Changwon Kang and Kyuyoung Song",

note = "Funding Information: We thank Yeonhee Choi and Jueun Chung for excellent technical assistance, Bernice Morrow for the earlier database search, and Se-yeon Weon for database assistance. We are grateful to Drs. Raju Kucherlapati, Oliver Smithies, Nobuyo Maeda, and Kyunghee Choi for helpful discussions. This work was supported by grants from the Asan Foundation through the Asan Institute for Life Sciences to K.S. and I.L. and Grant 913-0402-019-2 from the Korean Science and Engineering Foundation to IL.",

year = "1995",

doi = "10.1016/0888-7543(95)80008-A",

language = "English",

volume = "25",

pages = "660--666",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Identification and characterization of a human cDNA homologous to yeast SKI2

AU - Lee, Seong Gene

AU - Lee, Inchul

AU - Park, Sun H.

AU - Kang, Changwon

AU - Song, Kyuyoung

N1 - Funding Information: We thank Yeonhee Choi and Jueun Chung for excellent technical assistance, Bernice Morrow for the earlier database search, and Se-yeon Weon for database assistance. We are grateful to Drs. Raju Kucherlapati, Oliver Smithies, Nobuyo Maeda, and Kyunghee Choi for helpful discussions. This work was supported by grants from the Asan Foundation through the Asan Institute for Life Sciences to K.S. and I.L. and Grant 913-0402-019-2 from the Korean Science and Engineering Foundation to IL.

PY - 1995

Y1 - 1995

N2 - A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SKI2. The SKI2 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21.

AB - A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SKI2. The SKI2 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21.

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U2 - 10.1016/0888-7543(95)80008-A

DO - 10.1016/0888-7543(95)80008-A

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C2 - 7759100

AN - SCOPUS:0028922185

VL - 25

SP - 660

EP - 666

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -

Identification and characterization of a human cDNA homologous to yeast SKI2

Abstract

ASJC Scopus subject areas

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