Abstract
NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.
| Original language | English |
|---|---|
| Pages (from-to) | 529-534 |
| Number of pages | 6 |
| Journal | Biochemical and biophysical research communications |
| Volume | 391 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 Jan 1 |
| Externally published | Yes |
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Keywords
- Alternative splicing
- NELL2
- Neurite outgrowth
- Secretion
- Truncated NELL2
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Identification and characterization of a truncated isoform of NELL2. / Kim, Dong Gyu; Hwang, Eun Mi; Yoo, Jae Cheal; Kim, Eunju; Park, Nammi; Rhee, Sangmyung; Ha, Chang Man; Hong, Seong Geun; Park, Jae-Yong.
In: Biochemical and biophysical research communications, Vol. 391, No. 1, 01.01.2010, p. 529-534.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and characterization of a truncated isoform of NELL2
AU - Kim, Dong Gyu
AU - Hwang, Eun Mi
AU - Yoo, Jae Cheal
AU - Kim, Eunju
AU - Park, Nammi
AU - Rhee, Sangmyung
AU - Ha, Chang Man
AU - Hong, Seong Geun
AU - Park, Jae-Yong
PY - 2010/1/1
Y1 - 2010/1/1
N2 - NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.
AB - NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.
KW - Alternative splicing
KW - NELL2
KW - Neurite outgrowth
KW - Secretion
KW - Truncated NELL2
UR - http://www.scopus.com/inward/record.url?scp=72949109524&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72949109524&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.11.092
DO - 10.1016/j.bbrc.2009.11.092
M3 - Article
C2 - 19931511
AN - SCOPUS:72949109524
VL - 391
SP - 529
EP - 534
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -