Identification and characterization of a truncated isoform of NELL2

Dong Gyu Kim, Eun Mi Hwang, Jae Cheal Yoo, Eunju Kim, Nammi Park, Sangmyung Rhee, Chang Man Ha, Seong Geun Hong, Jae Yong Park

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalBiochemical and biophysical research communications
Volume391
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Keywords

  • Alternative splicing
  • NELL2
  • Neurite outgrowth
  • Secretion
  • Truncated NELL2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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