Identification and characterization of a truncated isoform of NELL2

Dong Gyu Kim; Eun Mi Hwang; Jae Cheal Yoo; Eunju Kim; Nammi Park; Sangmyung Rhee; Chang Man Ha; Seong Geun Hong; Jae Yong Park

doi:10.1016/j.bbrc.2009.11.092

Identification and characterization of a truncated isoform of NELL2

Dong Gyu Kim, Eun Mi Hwang, Jae Cheal Yoo, Eunju Kim, Nammi Park, Sangmyung Rhee, Chang Man Ha, Seong Geun Hong, Jae Yong Park

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

Original language	English
Pages (from-to)	529-534
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.11.092
Publication status	Published - 2010 Jan 1
Externally published	Yes

Keywords

Alternative splicing
NELL2
Neurite outgrowth
Secretion
Truncated NELL2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Identification and characterization of a truncated isoform of NELL2. / Kim, Dong Gyu; Hwang, Eun Mi; Yoo, Jae Cheal; Kim, Eunju; Park, Nammi; Rhee, Sangmyung; Ha, Chang Man; Hong, Seong Geun; Park, Jae Yong.

In: Biochemical and biophysical research communications, Vol. 391, No. 1, 01.01.2010, p. 529-534.

Research output: Contribution to journal › Article › peer-review

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abstract = "NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.",

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AU - Kim, Dong Gyu

AU - Hwang, Eun Mi

AU - Yoo, Jae Cheal

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AU - Park, Nammi

AU - Rhee, Sangmyung

AU - Ha, Chang Man

AU - Hong, Seong Geun

AU - Park, Jae Yong

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N2 - NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

AB - NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

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