Identification and characterization of a truncated isoform of NELL2

Dong Gyu Kim; Eun Mi Hwang; Jae Cheal Yoo; Eunju Kim; Nammi Park; Sangmyung Rhee; Chang Man Ha; Seong Geun Hong; Jae Yong Park

doi:10.1016/j.bbrc.2009.11.092

Identification and characterization of a truncated isoform of NELL2

Dong Gyu Kim, Eun Mi Hwang, Jae Cheal Yoo, Eunju Kim, Nammi Park, Sangmyung Rhee, Chang Man Ha, Seong Geun Hong, Jae Yong Park

School of Biosystem and Biomedical Science

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

Original language	English
Pages (from-to)	529-534
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.11.092
Publication status	Published - 2010 Jan 1

Keywords

Alternative splicing
NELL2
Neurite outgrowth
Secretion
Truncated NELL2

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kim, D. G., Hwang, E. M., Yoo, J. C., Kim, E., Park, N., Rhee, S., Ha, C. M., Hong, S. G., & Park, J. Y. (2010). Identification and characterization of a truncated isoform of NELL2. Biochemical and biophysical research communications, 391(1), 529-534. https://doi.org/10.1016/j.bbrc.2009.11.092

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title = "Identification and characterization of a truncated isoform of NELL2",

abstract = "NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.",

keywords = "Alternative splicing, NELL2, Neurite outgrowth, Secretion, Truncated NELL2",

author = "Kim, {Dong Gyu} and Hwang, {Eun Mi} and Yoo, {Jae Cheal} and Eunju Kim and Nammi Park and Sangmyung Rhee and Ha, {Chang Man} and Hong, {Seong Geun} and Park, {Jae Yong}",

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T1 - Identification and characterization of a truncated isoform of NELL2

AU - Kim, Dong Gyu

AU - Hwang, Eun Mi

AU - Yoo, Jae Cheal

AU - Kim, Eunju

AU - Park, Nammi

AU - Rhee, Sangmyung

AU - Ha, Chang Man

AU - Hong, Seong Geun

AU - Park, Jae Yong

PY - 2010/1/1

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N2 - NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

AB - NELL2 is a neuron-specific secreted glycoprotein containing an N-terminal thrombospondin I-like domain (TSP-N). In this study, we describe NELL2-Tsp, a novel alternative splice variant of rat NELL2. NELL2-Tsp uses an alternate stop codon resulting in a C-terminal truncated form of NELL2, containing a signal peptide and a TSP-N domain. NELL2-Tsp is a glycosylated protein specifically expressed in brain tissue. NELL2-Tsp and NELL2 are secreted, likely due to the putative signal peptide. However, due to the truncation, the secreted portion of NELL2-Tsp is smaller than that of NELL2. Immunoprecipitation analysis confirmed that NELL2-Tsp was able to associate with NELL2 and with itself. In addition, expression of NELL2-Tsp notably reduced secretion of NELL2 and inhibited NELL2-mediated neurite outgrowth. These results suggest that NELL2-Tsp may act as a negative regulator of wild-type NELL2.

KW - Alternative splicing

KW - NELL2

KW - Neurite outgrowth

KW - Secretion

KW - Truncated NELL2

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