Abstract
Several Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition, C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
| Original language | English |
|---|---|
| Pages (from-to) | 129-137 |
| Number of pages | 9 |
| Journal | Crop Protection |
| Volume | 32 |
| DOIs | |
| Publication status | Published - 2012 Feb 1 |
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Keywords
- Antagonistic rhizobacteria
- Biological control
- Chryseobacterium wanjuense
- Phytophthora capsici
- Root colonization
- Swarming motility
ASJC Scopus subject areas
- Agronomy and Crop Science
Cite this
Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper. / Kim, Hye Sook; Sang, Mee Kyung; Jung, Ho Won; Jeun, Yong Chull; Myung, Inn Shik; Kim, Ki Deok.
In: Crop Protection, Vol. 32, 01.02.2012, p. 129-137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper
AU - Kim, Hye Sook
AU - Sang, Mee Kyung
AU - Jung, Ho Won
AU - Jeun, Yong Chull
AU - Myung, Inn Shik
AU - Kim, Ki Deok
PY - 2012/2/1
Y1 - 2012/2/1
N2 - Several Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition, C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
AB - Several Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition, C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
KW - Antagonistic rhizobacteria
KW - Biological control
KW - Chryseobacterium wanjuense
KW - Phytophthora capsici
KW - Root colonization
KW - Swarming motility
UR - http://www.scopus.com/inward/record.url?scp=82255180284&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82255180284&partnerID=8YFLogxK
U2 - 10.1016/j.cropro.2011.10.018
DO - 10.1016/j.cropro.2011.10.018
M3 - Article
AN - SCOPUS:82255180284
VL - 32
SP - 129
EP - 137
JO - Crop Protection
JF - Crop Protection
SN - 0261-2194
ER -