Identification and characterization of the Vibrio vulnificus rtxA essential for cytotoxicity in vitro and virulence in mice

Hyun Lee Jeong, Won Kim Myung, Sik Kim Byoung, Min Kim Seung, Cheol Lee Byung, Sung Kim Tae, Ho Choi Sang

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalJournal of Microbiology
Volume45
Issue number2
Publication statusPublished - 2007

Keywords

  • Cytotoxicity
  • RtxA
  • Vibrio vulnificus
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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