Identification and functional analysis of Vibrio vulnificus SmcR, a novel global regulator

Jeong Hyun Lee, Jee Eun Rhee, Uryung Park, Hyun Mok Ju, Byung Cheol Lee, Tae Sung Kim, Hye Sook Jeong, Sang Ho Choi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Recently, quorum sensing has been implicated as an important global regulator controlling the production of numerous virulence factors such as capsular polysaccharides in bacterial pathogens. The nucleotide and deduced amino acid sequences of smcR, a homolog of V. harveyi luxR identified from V. vulnificus ATCC29307, were analyzed. The amino acid sequence of SmcR from V. vulnificus was 72 to 92% similar to those of LuxR homologs from Vibrio spp. Functions of SmcR were assessed by the construction of an isogenic mutant, whose smcR gene was inactivated by allelic exchanges, and by evaluating its phenotype changes in vitro and in mice. The disruption of smcR resulted in a significant alteration in biofilm formation, in type of colony morphology, and in motility. When compared with the wild-type, the smcR mutant exhibited reduced survival under adverse conditions, such as acidic pH and hyperosmotic stress. The smcR mutant exhibited decreased cytotoxic activity toward INT 407 cells in vitro. Furthermore, the intraperitoneal LD50 of the smcR mutant was approximately 102 times higher than that of parental wild-type. Therefore, it appears that SmcR is a novel global regulator, controlling numerous genes contributing to the pathogenesis as well as survival of V. vulnificus.

Original languageEnglish
Pages (from-to)325-334
Number of pages10
JournalJournal of microbiology and biotechnology
Volume17
Issue number2
Publication statusPublished - 2007 Feb

Keywords

  • Novel global regulator
  • Vibrio vulnificus
  • smcR

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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  • Cite this

    Lee, J. H., Rhee, J. E., Park, U., Ju, H. M., Lee, B. C., Kim, T. S., Jeong, H. S., & Choi, S. H. (2007). Identification and functional analysis of Vibrio vulnificus SmcR, a novel global regulator. Journal of microbiology and biotechnology, 17(2), 325-334.