Abstract
Galactinol and rafinose accumulation in plants is associated with stressful environmental conditions such as cold, heat, or dehydration by the action of galactinols synthase (GolS) in the raffinose family of oligosaccharides biosynthetic pathway from UDP-galactose. Moreover, several reports mentioned that GolS transcription is up regulated by various environmental stresses like cold, heat, dehydration. Therefore, to determine whether MoGolS1 was induced with the abiotic stress we analyzed the expression pattern of the gene under various abiotic stresses like heat, cold, abscisic acid, sucrose and salt concentration in the lemon balm plants grown in standard MS medium. The MoGolS1 gene was 981-bp in length encoding 326 amino acids in its sequence and shared 77 and 76% sequence similarity with Arabidopsis thaliana galactinol synthase4 (AtGolS4) and AtGolS1 genes respectively. The MoGolS1 gene was strongly expressed by the abiotic stress induced by sucrose, ABA or heat shock. It was also expressed in responses to cold, manitol and NaCl. MoGolS1 gene induction with various stresses may be possible for itscrucial function in abiotic stress tolerance in plants, providing a good engineering target for genetic engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 244-251 |
| Number of pages | 8 |
| Journal | Journal of Applied Biological Chemistry |
| Volume | 54 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 Dec 1 |
Fingerprint
Keywords
- Abiotic stress
- Abscisic acid
- Galactinol synthase
- Melissa officinalis
- Sucrose
ASJC Scopus subject areas
- Organic Chemistry
- Bioengineering
Cite this
Identification and functional characterization of the GALACTINOL SYNTHASE (MoGolS1) gene in melissa officinalis plants. / Kim, Jun Hyeok; Hossain, Acktar Mohammad; Kim, Nahyun; Lee, Dongho; Lee, Hojoung.
In: Journal of Applied Biological Chemistry, Vol. 54, No. 4, 01.12.2011, p. 244-251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification and functional characterization of the GALACTINOL SYNTHASE (MoGolS1) gene in melissa officinalis plants
AU - Kim, Jun Hyeok
AU - Hossain, Acktar Mohammad
AU - Kim, Nahyun
AU - Lee, Dongho
AU - Lee, Hojoung
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Galactinol and rafinose accumulation in plants is associated with stressful environmental conditions such as cold, heat, or dehydration by the action of galactinols synthase (GolS) in the raffinose family of oligosaccharides biosynthetic pathway from UDP-galactose. Moreover, several reports mentioned that GolS transcription is up regulated by various environmental stresses like cold, heat, dehydration. Therefore, to determine whether MoGolS1 was induced with the abiotic stress we analyzed the expression pattern of the gene under various abiotic stresses like heat, cold, abscisic acid, sucrose and salt concentration in the lemon balm plants grown in standard MS medium. The MoGolS1 gene was 981-bp in length encoding 326 amino acids in its sequence and shared 77 and 76% sequence similarity with Arabidopsis thaliana galactinol synthase4 (AtGolS4) and AtGolS1 genes respectively. The MoGolS1 gene was strongly expressed by the abiotic stress induced by sucrose, ABA or heat shock. It was also expressed in responses to cold, manitol and NaCl. MoGolS1 gene induction with various stresses may be possible for itscrucial function in abiotic stress tolerance in plants, providing a good engineering target for genetic engineering.
AB - Galactinol and rafinose accumulation in plants is associated with stressful environmental conditions such as cold, heat, or dehydration by the action of galactinols synthase (GolS) in the raffinose family of oligosaccharides biosynthetic pathway from UDP-galactose. Moreover, several reports mentioned that GolS transcription is up regulated by various environmental stresses like cold, heat, dehydration. Therefore, to determine whether MoGolS1 was induced with the abiotic stress we analyzed the expression pattern of the gene under various abiotic stresses like heat, cold, abscisic acid, sucrose and salt concentration in the lemon balm plants grown in standard MS medium. The MoGolS1 gene was 981-bp in length encoding 326 amino acids in its sequence and shared 77 and 76% sequence similarity with Arabidopsis thaliana galactinol synthase4 (AtGolS4) and AtGolS1 genes respectively. The MoGolS1 gene was strongly expressed by the abiotic stress induced by sucrose, ABA or heat shock. It was also expressed in responses to cold, manitol and NaCl. MoGolS1 gene induction with various stresses may be possible for itscrucial function in abiotic stress tolerance in plants, providing a good engineering target for genetic engineering.
KW - Abiotic stress
KW - Abscisic acid
KW - Galactinol synthase
KW - Melissa officinalis
KW - Sucrose
UR - http://www.scopus.com/inward/record.url?scp=84862940720&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862940720&partnerID=8YFLogxK
U2 - 10.3839/jabc.2011.040
DO - 10.3839/jabc.2011.040
M3 - Article
AN - SCOPUS:84862940720
VL - 54
SP - 244
EP - 251
JO - Journal of Applied Biological Chemistry
JF - Journal of Applied Biological Chemistry
SN - 1976-0442
IS - 4
ER -