Identification and functional characterization of the GALACTINOL SYNTHASE (MoGolS1) gene in melissa officinalis plants

Jun Hyeok Kim, Acktar Mohammad Hossain, Nahyun Kim, Dongho Lee, Hojoung Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Galactinol and rafinose accumulation in plants is associated with stressful environmental conditions such as cold, heat, or dehydration by the action of galactinols synthase (GolS) in the raffinose family of oligosaccharides biosynthetic pathway from UDP-galactose. Moreover, several reports mentioned that GolS transcription is up regulated by various environmental stresses like cold, heat, dehydration. Therefore, to determine whether MoGolS1 was induced with the abiotic stress we analyzed the expression pattern of the gene under various abiotic stresses like heat, cold, abscisic acid, sucrose and salt concentration in the lemon balm plants grown in standard MS medium. The MoGolS1 gene was 981-bp in length encoding 326 amino acids in its sequence and shared 77 and 76% sequence similarity with Arabidopsis thaliana galactinol synthase4 (AtGolS4) and AtGolS1 genes respectively. The MoGolS1 gene was strongly expressed by the abiotic stress induced by sucrose, ABA or heat shock. It was also expressed in responses to cold, manitol and NaCl. MoGolS1 gene induction with various stresses may be possible for itscrucial function in abiotic stress tolerance in plants, providing a good engineering target for genetic engineering.

Original languageEnglish
Pages (from-to)244-251
Number of pages8
JournalJournal of Applied Biological Chemistry
Volume54
Issue number4
DOIs
Publication statusPublished - 2011 Dec 1

Fingerprint

inositol 1-alpha-galactosyltransferase
Genes
Sugar (sucrose)
Dehydration
Sucrose
Uridine Diphosphate Galactose
Raffinose
Genetic engineering
Oligosaccharides
Abscisic Acid
Transcription
Amino acids
Salts
Amino Acids
Acids
Hot Temperature
6 beta-galactinol

Keywords

  • Abiotic stress
  • Abscisic acid
  • Galactinol synthase
  • Melissa officinalis
  • Sucrose

ASJC Scopus subject areas

  • Organic Chemistry
  • Bioengineering

Cite this

Identification and functional characterization of the GALACTINOL SYNTHASE (MoGolS1) gene in melissa officinalis plants. / Kim, Jun Hyeok; Hossain, Acktar Mohammad; Kim, Nahyun; Lee, Dongho; Lee, Hojoung.

In: Journal of Applied Biological Chemistry, Vol. 54, No. 4, 01.12.2011, p. 244-251.

Research output: Contribution to journalArticle

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