Identification and initial characterizations of free, glycosylated, and phosphorylated ceramides of Paramecium

Edna S. Kaneshiro, Koka Jayasimhulu, Dong Geun Sul, Joseph A. Erwin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Paremecium tetraurelia contains high concentrations of six ethanolamine sphingolipids in the cell surface membrane surrounding somatic cilia. Three have phosphoryl groups and the other three have phosphonyl groups, and each contains either dihydrosphingosine, sphingosine, or phytosphingosine. In the present study, free, phosphorylated, and three major glycosylated ceramides were identified in the neutral sphingolipid fraction of this organism when it was grown on a crude medium. Individual lipids were characterized by specific staining on thin-layer chromatographic plates and the fatty acids derived from them were identified by gas-liquid chromatography and mass spectrometry. Unlike the esterified fatty acid composition of the ciliate's phospholipids, which have only small amounts of fatty acids greater than 20 carbons in length, the neutral sphingolipids mainly contain fatty acids greater than 22 carbons in length as well as high concentrations of long chain hydroxy fatty acids. The sugars, glucose, galactose, and fructose, were identified in total neutral sphingolipid fraction. The long chain bases in the neutral sphingolipid fraction were mainly C18 compounds and were identified as C18 dihydrosphingosine, C20 sphingosine, and isomers of C18 sphingosine. Phytosphingosine was not detected in the neutral sphingolipid fraction obtained from whole cells. Because most of the cell's phytosphingosine-containing ethanolamine sphingolipids are in cilia, the inability to detect phytosphingosine as part of putative precursor ceramide compounds suggests that conversions of the long chain base moiety of ethanolamine phospho- and phosphonosphingolipids occur in situ in the ciliary membrane.

Original languageEnglish
Pages (from-to)2399-2410
Number of pages12
JournalJournal of Lipid Research
Volume38
Issue number12
Publication statusPublished - 1997 Dec 1
Externally publishedYes

Fingerprint

phytosphingosine
Paramecium
Sphingolipids
Ceramides
Fatty Acids
Ethanolamine
Sphingosine
Cilia
Carbon
Membranes
Hydroxy Acids
Liquid chromatography
Fructose
Galactose
Sugars
Isomers
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Phospholipids

Keywords

  • Cilia
  • Fatty acids
  • Glycolipids
  • Long chain bases
  • Sugars

ASJC Scopus subject areas

  • Endocrinology

Cite this

Identification and initial characterizations of free, glycosylated, and phosphorylated ceramides of Paramecium. / Kaneshiro, Edna S.; Jayasimhulu, Koka; Sul, Dong Geun; Erwin, Joseph A.

In: Journal of Lipid Research, Vol. 38, No. 12, 01.12.1997, p. 2399-2410.

Research output: Contribution to journalArticle

Kaneshiro, Edna S. ; Jayasimhulu, Koka ; Sul, Dong Geun ; Erwin, Joseph A. / Identification and initial characterizations of free, glycosylated, and phosphorylated ceramides of Paramecium. In: Journal of Lipid Research. 1997 ; Vol. 38, No. 12. pp. 2399-2410.
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