Identification of a ζ-crystallin (quinone reductase)-like 1 gene (CRYZL1) mapped to human chromosome 21q22.1

Min Young Kim, Hye Kyung Lee, Jung Sun Park, Sun Hwa Park, Hyuk Bang Kwon, Jaemog Soh

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    To identify a new gene(s) located on the yeast artificial chromosome (YAC) clone D142H8 that was mapped to human chromosome 21q22.1, purified YAC DNA from the clone was utilized directly as a probe to screen a human brain eDNA library after the suppression of human repetitive DNA. One eDNA clone hybridizing specifically to the YAC D142H8 DNA was identified. The clone has an insert of 1341 bp and the longest open reading frame of 349 amino acids. A search of GenBank revealed that the clone has a high degree of homology to ζ-crystallin (quinone reductase) at the amino acid level, and its nucleotide sequence represents the expressed sequence from the 50-kb segment of the human chromosome 21q11.1. Thus a new gene was named CRYZL1 (ζ-crystalline- like 1). Genom Southern blot with total human and yeast DNAs suggests that CRYZL1 might be a single-copy gene. The fluorescence in situ hybridization procedure was applied, and the results showed that the gene mapped to the human chromosome 21q22.1 subband. The CRYZL1 mRNA was expressed in heart, brain, skeletal muscle, kidney, pancreas, liver, and lungs but at different levels in different tissues.

    Original languageEnglish
    Pages (from-to)156-159
    Number of pages4
    JournalGenomics
    Volume57
    Issue number1
    DOIs
    Publication statusPublished - 1999 Apr 1

    ASJC Scopus subject areas

    • Genetics

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