Identification of a new functional target of haloperidol metabolite: Implications for a receptor-independent role of 3-(4-fluorobenzoyl) propionic acid

Hyeon Soo Kim, Minseok Song, Sanatombi Yumkham, Jang Hyun Choi, Taehoon Lee, Joseph Kwon, Sung Jae Lee, Jong In Kim, Kang Woo Lee, Pyung Lim Han, Seung Woo Shin, Ja Hyun Baik, Yong Sik Kim, Sung Ho Ryu, Pann Ghill Suh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Haloperidol, a dopamine D2 receptor blocker, is a classical neuroleptic drug that elicits extrapyramidal symptoms. Its metabolites include 3-(4-fluorobenzoyl) propionic acid (FBPA) and 4-(4-chlorophenyl)-4-piperidinol (CPHP). Until now, the biological significance of these metabolites has remained largely unknown. Here, we report that the administration of FBPA to mice effected a suppression of locomotor activity and induced catalepsy in a manner similar to that observed with haloperidol, whereas CPHP had no significant effects. Neither of these two metabolites, however, exhibited any ability to bind to the dopamine D2 receptor. FBPA blocked dopamine-induced extracellular signal-regulated kinase 1/2 phosphorylation, and it specifically affected mitogen-activated protein kinase kinase (MEK)1/2 activity in hippocampal HN33 cells. Moreover, FBPA was capable of direct interaction with MEK1/2, and inhibited its activity in vitro. We demonstrated the generation of haloperidol metabolites within haloperidol-treated cells by mass spectrometric analyses. Collectively, our results confirm the biological activity of FBPA, and provide initial clues as to the receptor-independent role of haloperidol.

Original languageEnglish
Pages (from-to)458-469
Number of pages12
JournalJournal of Neurochemistry
Volume99
Issue number2
DOIs
Publication statusPublished - 2006 Oct

Keywords

  • Antipsychotic drug
  • Dopamine D2 receptor
  • Haloperidol
  • Metabolite
  • Mitogen-activated protein kinase kinase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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  • Cite this

    Kim, H. S., Song, M., Yumkham, S., Choi, J. H., Lee, T., Kwon, J., Lee, S. J., Kim, J. I., Lee, K. W., Han, P. L., Shin, S. W., Baik, J. H., Kim, Y. S., Ryu, S. H., & Suh, P. G. (2006). Identification of a new functional target of haloperidol metabolite: Implications for a receptor-independent role of 3-(4-fluorobenzoyl) propionic acid. Journal of Neurochemistry, 99(2), 458-469. https://doi.org/10.1111/j.1471-4159.2006.04108.x