Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities

Ssang Goo Cho; Jin Woo Kim; Yong Hee Lee; Hyun Sub Hwang; Mi Sung Kim; Kanghyun Ryoo; Myung Jin Kim; Kyung Tae Noh; Eun Kyung Kim; Jun Ho Cho; Kyoung Wan Yoon; Eun Gyung Cho; Hee Sae Park; Sung Wook Chi; Min Jae Lee; Sang Sun Kang; Hidenori Ichijo; Eui Ju Choi

doi:10.1083/jcb.200303003

Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities

Ssang Goo Cho, Jin Woo Kim, Yong Hee Lee, Hyun Sub Hwang, Mi Sung Kim, Kanghyun Ryoo, Myung Jin Kim, Kyung Tae Noh, Eun Kyung Kim, Jun Ho Cho, Kyoung Wan Yoon, Eun Gyung Cho, Hee Sae Park, Sung Wook Chi, Min Jae Lee, Sang Sun Kang, Hidenori Ichijo, Eui Ju Choi

Research output: Contribution to journal › Article

38 Citations (Scopus)

Abstract

Diverse stimuli initiate the activation of apoptotic signaling pathways that often causes nuclear DNA fragmentation. Here, we report a new antiapoptotic protein, a caspase-activated DNase (CAD) inhibitor that interacts with ASK1 (CIIA). CIIA, by binding to apoptosis signal-regulating kinase 1 (ASK1), inhibits oligomerization-induced ASK1 activation. CIIA also associates with CAD and inhibits the nuclease activity of CAD without affecting caspase-3-mediated ICAD cleavage. Overexpressed CIIA reduces H₂O ₂- and tumor necrosis factor-α-induced apoptosis. CIIA antisense oligonucleotides, which abolish expression of endogenous CIIA in murine L929 cells, block the inhibitory effect of CIIA on ASK1 activation, deoxyribonucleic acid fragmentation, and apoptosis. These findings suggest that CIIA is an endogenous antagonist of both ASK1 - and CAD-mediated signaling.

Original language	English
Pages (from-to)	71-81
Number of pages	11
Journal	Journal of Cell Biology
Volume	163
Issue number	1
DOIs	https://doi.org/10.1083/jcb.200303003
Publication status	Published - 2003 Oct 13

Keywords

Apoptosis
Apoptosis signal-regulating kinase 1
C-Jun NH-terminal kinase
Caspase-activated DNase
Stress-activated protein kinase

ASJC Scopus subject areas

Cell Biology

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Cho, S. G., Kim, J. W., Lee, Y. H., Hwang, H. S., Kim, M. S., Ryoo, K., Kim, M. J., Noh, K. T., Kim, E. K., Cho, J. H., Yoon, K. W., Cho, E. G., Park, H. S., Chi, S. W., Lee, M. J., Kang, S. S., Ichijo, H., & Choi, E. J. (2003). Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities. Journal of Cell Biology, 163(1), 71-81. https://doi.org/10.1083/jcb.200303003

Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities. / Cho, Ssang Goo; Kim, Jin Woo; Lee, Yong Hee; Hwang, Hyun Sub; Kim, Mi Sung; Ryoo, Kanghyun; Kim, Myung Jin; Noh, Kyung Tae; Kim, Eun Kyung; Cho, Jun Ho; Yoon, Kyoung Wan; Cho, Eun Gyung; Park, Hee Sae; Chi, Sung Wook; Lee, Min Jae; Kang, Sang Sun; Ichijo, Hidenori; Choi, Eui Ju.

In: Journal of Cell Biology, Vol. 163, No. 1, 13.10.2003, p. 71-81.

Research output: Contribution to journal › Article

Cho, Ssang Goo ; Kim, Jin Woo ; Lee, Yong Hee ; Hwang, Hyun Sub ; Kim, Mi Sung ; Ryoo, Kanghyun ; Kim, Myung Jin ; Noh, Kyung Tae ; Kim, Eun Kyung ; Cho, Jun Ho ; Yoon, Kyoung Wan ; Cho, Eun Gyung ; Park, Hee Sae ; Chi, Sung Wook ; Lee, Min Jae ; Kang, Sang Sun ; Ichijo, Hidenori ; Choi, Eui Ju. / Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities. In: Journal of Cell Biology. 2003 ; Vol. 163, No. 1. pp. 71-81.

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