Identification of a novel human Rad51 variant that promotes DNA strand exchange

Jung Young Park, Han Wook Yoo, Bok Ryang Kim, Raekil Park, Sang-Yun Choi, Youngho Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rad51 plays a key role in the repair of DNA double-strand breaks through homologous recombination, which is the central process in the maintenance of genomic integrity. Five paralogs of the human Rad51 gene (hRad51) have been identified to date, including hRad51B, hRad51C, hRad51D, Xrcc2 and Xrcc3. In searches of additional hRad51 paralogs, we identified a novel hRad51 variant that lacked the sequence corresponding to exon 9 (hRad51-Δex9). The expected amino acid sequence of hRad51-Δex9 showed a frame-shift at codon 259, which resulted in a truncated C-terminus. RT-PCR analysis revealed that both hRad51 and hRad51-Δex9 were prominently expressed in the testis, but that there were subtle differences in tissue specificity. The hRad51-Δex9 protein was detected as a 31-kDa protein in the testis and localized at the nucleus. In addition, the hRad51-Δex9 protein showed a DNA-strand exchange activity comparable to that of hRad51. Taken together, these results indicate that hRad51-Δex9 promotes homologous pairing and DNA strand exchange in the nucleus, suggesting that alternative pathways in hRad51- or hRad51-Δex9-dependent manners exist for DNA recombination and repair.

Original languageEnglish
Pages (from-to)3226-3234
Number of pages9
JournalNucleic Acids Research
Volume36
Issue number10
DOIs
Publication statusPublished - 2008 Jun 1

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Genes
DNA
Repair
Testis
Proteins
Recombinational DNA Repair
Organ Specificity
Double-Stranded DNA Breaks
Homologous Recombination
Exons
Codon
DNA Repair
Amino Acid Sequence
Tissue
Amino Acids
Maintenance
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of a novel human Rad51 variant that promotes DNA strand exchange. / Park, Jung Young; Yoo, Han Wook; Kim, Bok Ryang; Park, Raekil; Choi, Sang-Yun; Kim, Youngho.

In: Nucleic Acids Research, Vol. 36, No. 10, 01.06.2008, p. 3226-3234.

Research output: Contribution to journalArticle

Park, Jung Young ; Yoo, Han Wook ; Kim, Bok Ryang ; Park, Raekil ; Choi, Sang-Yun ; Kim, Youngho. / Identification of a novel human Rad51 variant that promotes DNA strand exchange. In: Nucleic Acids Research. 2008 ; Vol. 36, No. 10. pp. 3226-3234.
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